Lipase (gene EaLIP27) from Exiguobacterium sp.

Enzyme Description

Species
Exiguobacterium sp. β†—
Extremophile
No but"cold-adapted" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 249 amino acids
MKQSKWYAFLTGAVLLTGIALYGLWIGYQDIVNPPERTLSVSEDERPEPEGDIYVALGDSLTRGVGSTSGAGYVQPVSEGLEDESLRTQNLAVSGARTEDLLAQLEQPEVRRTIESARYITLTIGGNDLFNRGENVDNFESVDIQQVVTDAKTNLETIFSEIRALNDSATIVYIGLYNPFQNDENGQAFNQLILDWNASAKQLANAQNIDVIDPFAYISDLSRDLATDQFHPSDRTYEKFANDVLFVLE
Yanpo Li et al. (2025) β€” Characterization of Cold-Adapted Lipase from Exiguobacterium sp. and Its Cold Adaptation Mechanism
Journal of Agricultural and Food Chemistry  Β· doi:10.1021/acs.jafc.4c11222 β†—  Β· Activity - Classical
10 measurements ▼
Database ID
UniParc: UPI003366F27D β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 30% 100.0 56.2 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 30% 100.0 86.2 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent 1-Butanol 30% 100.0 49.6 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Hexanenitrile 30% 100.0 90.6 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isopropanol 30% 100.0 81.5 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 30% 100.0 82.2 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 30% 100.0 93.5 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Heptane 30% 100.0 122.2 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 30% 100.0 127.7 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100.0 89.3 % 25 mM phosphate buffer 7 30Β°C ? mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” 150 rpm Classical aqueous control (in %) | Apparently wrong substrate given in article , probably pNPP is p-Nitrophenyl palmitate

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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