Lipase B from Ustilago maydis
Enzyme Description
Sequence
MKTTSVISALVTLASIIRAAPLASSDPAFSTPKATLDAGLECQTGSPSSQTKPILLVPGTGANGTQTFDSSWIPLSAKLGFSPCWISPPPFMLNDSQVNVEYIVNAVQTLYAGSGSKKVPVLTWSQGGLATQWALTFFPSIRNQVDRLMAFAPDYKGTIEAGLLSTFGLASQSVWQQQAGSAFVTALKNAGGLTSFVPTTNLYSFFDEIVQPQVFNSDADSSYLGNSKNIQAQTVCGGFFVIDHAGSLTSQFSYVVGKSALTSSSGVANSADYSSKDCKASPADDLSAKQKADASALLFVAAGNLLAGPKQNCEPDLKPYARQFAVGKKTCSGTIN
Marcela Robles-Machuca et al. (2025)
β Further Characterization of Lipase B from Ustilago maydis Expressed in Pichia pastoris: a Member of the Candida antarctica Lipase B-like Superfamily
Applied Biochemistry and Biotechnology
Β· doi:10.1007/s12010-024-05166-0 β
Β· Stability - Incubation
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Database ID
UniProt: A0A0D1CES2 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host yeast Pichia pastoris X-33
Experimental Data (10 measurements)
10 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 30% | 100 | 42 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Methanol | 30% | 100 | 25 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Acetonitrile | 30% | 100 | 0 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Acetone | 30% | 100 | 38 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Tetrahydrofuran (THF) | 30% | 100 | 2 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | 1-Butanol | 30% | 100 | 15 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | tert-Butanol | 30% | 100 | 29 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Toluene | 30% | 100 | 127 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | n-Hexane | 30% | 100 | 125 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
| Stability - Incubation | Activity after incubation (2 hours at 30Β°C), measured by pH shift caused by fatty acid release (p-nitrophenol indicator) in aqueous phase | Isooctane | 30% | 100 | 115 | % | 2.5 mM 4-morpholinepropane- sulfonic acid buffer incubation, 2.5 mM 4-morpholinepropane- sulfonic acid buffer, 150 mM NaCl, 6 mM CaCl2, 0.5 MM sodium taurodeoxycholate, 3 mg/ml Ξ²-cyclodextrin | Incubation: 7.2, Assay: 7 | Incubation: 30Β°C, Assay: ? | 50 mM Tributyrin | 3-butyric acid , glycerol | β | Incubation: 1000 rpm | Non-incubated control (in %), assay in aqueous phase | Clear about assay in aqueous phase ("After incubation, the organic phase was separated by centrifugation at maxi- mum speed for 15 min for samples containing hydrophobic solvents, retaining only the aqueous phase where the enzyme was present. For hydrophilic solvent samples, a fraction of the solvent-buffer mixture was taken directly. In both cases, the sample was then diluted tenfold in 2.5 mmol Lβ1 MOPS buffer at pH 7") |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.