Lipase (gene LipA) from Bacillus licheniformis IBRL-CHS2

Enzyme Description

Species
Bacillus licheniformis IBRL-CHS2 β†—
Extremophile
No
EC Number
3.1.1.3 β†—

Sequence

Length: 205 amino acids
MRRHSFLSILLILCMLSVVSVFSFRPSAASAASHNPVVMVHGIGGADYNFIGIKSYLQSQGWTSSELYAINFIDKTGNNINNAPRLSEYIKRVLNQTGASKVDIVAHSMGGANTLYYIKNLDGADKVGHVVTLGGANRLVTNTAPQNDKISYTSIYSTSDYIVLNSLSKLDGANNVQISGVSHVGLLFSSKVNALIKDGLTASGK
Ammar Khazaal Kadhim Almansoori et al. (2024) β€” Characterization of a novel subfamily 1.4 lipase from Bacillus licheniformis IBRL-CHS2: Cloning and expression optimization
PLOS ONE  Β· doi:10.1371/journal.pone.0314556 β†—  Β· Activity + Stability - Incubation
20 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25% 100 139 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 25% 100 181 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Heptane 25% 100 83 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 25% 100 105 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Chloroform 25% 100 4 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Diethyl Ether 25% 100 38 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 25% 100 52 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 25% 100 74 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 25% 100 68 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (24 hours at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 25% 100 114 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25% 100 161 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Isooctane 25% 100 192 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Heptane 25% 100 102 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent n-Hexane 25% 100 159 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Chloroform 25% 100 28 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Diethyl Ether 25% 100 55 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetonitrile 25% 100 76 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Ethanol 25% 100 97 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Acetone 25% 100 61 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (1 hour at 35Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent Methanol 25% 100 114 % Incubation: ?, Assay: 100 mM phosphate buffer, 0.1% (w/v) polyvinyl alcohol, 0.01% Triton X-100 Incubation: ?, Assay: 7 Incubation: 35Β°C, Assay: 35Β°C 0.5 mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” Incubation: 100 rpm Water-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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