Arylsulfate sulfotransferase from Desulfitobacterium hafniense
Enzyme Description
Sequence
MVDYEYCPHIITKQNELEKAFMEKFEAGNYSLENPLVILNPYGVAPLTAMILFNTPIACEAKIIVKGKEHPGDIQHTFPAATRHILPVYGLYGGYKNTIKIILSTSSSNTITIQTEPLPKSVAIPSSIKTTSEYMGNNLMFLSPSMASLTVGYDYAGDVRWYCSLNVCFDLKRMPNGHLLIGTGRLVKVPYYTSGLYEMAFSGKIFKEFILPGGYHHDQFVMEDGNLLVLTQDFYADTVEDVCVLIDPHSGDILKKWDFKNILPQDVAGSGTQTAHDWFHNNAVWYDNRTNSLTLSGRHQDVIINIDYETGGLNWMIGDPEGWPQELVDKYFFTPAGDGEFDWQYEQHGCVVLPDGDIMVFDNGHYRSKNKENYRLNKDNFSRGVRYRIDTERMTIQQIWQYGKERGADFFSPFICNVEYYDEGHYMVHSGGIGYENGKPCEGLAVGKSKNPKFKDNVYTLNSITCELVNDELVYELQVPANFYRAEKLPIYYANEVAELCEGQRLGQFVETQTTRMKIKALETKEPIPEHYQAKIVEEEDRFCLNAIYESGETAQLLLVSESDEILRYPINTVAQAYGAMCVGTFQKSDPRNVDTFVTKTGLSGKYQVKLVAEDKIYETGVTISA
Isabel Oroz-Guinea et al. (2024)
β Biocatalytic sulfation of aromatic and aliphatic alcohols catalyzed by arylsulfate sulfotransferases
Applied Microbiology and Biotechnology
Β· doi:10.1007/s00253-024-13354-5 β
Β· Activity - Classical
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Database ID
β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (6 measurements)
6 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 10% | 100.0 | 41.3 | % | 50 mM Tris-HCl buffer | 8 | 30Β°C | 1 mM p-Nitrophenyl sulfate, 1 mM Phenol | p-Nitrophenol , phenyl sulfate | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Acetone | 10% | 100.0 | 21.0 | % | 50 mM Tris-HCl buffer | 8 | 30Β°C | 1 mM p-Nitrophenyl sulfate, 1 mM Phenol | p-Nitrophenol , phenyl sulfate | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Methanol | 10% | 100.0 | 64.2 | % | 50 mM Tris-HCl buffer | 8 | 30Β°C | 1 mM p-Nitrophenyl sulfate, 1 mM Phenol | p-Nitrophenol , phenyl sulfate | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Isopropanol | 10% | 100.0 | 62.3 | % | 50 mM Tris-HCl buffer | 8 | 30Β°C | 1 mM p-Nitrophenyl sulfate, 1 mM Phenol | p-Nitrophenol , phenyl sulfate | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 10% | 100.0 | 8.5 | % | 50 mM Tris-HCl buffer | 8 | 30Β°C | 1 mM p-Nitrophenyl sulfate, 1 mM Phenol | p-Nitrophenol , phenyl sulfate | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent | 1,2-Dimethoxyethane | 10% | 100.0 | 38.8 | % | 50 mM Tris-HCl buffer | 8 | 30Β°C | 1 mM p-Nitrophenyl sulfate, 1 mM Phenol | p-Nitrophenol , phenyl sulfate | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.