Carboxylesterase from Pyrobaculum calidifontis VA1
Enzyme Description
Species
Extremophile
Yes
"hyperthermophilic" organism
EC Number
Sequence
MPLSPILRQILQQLAAQLQFRPDMDVKTVREQFEKSSLILVKMANEPIHRVEDITIPGRGGPIRARVYRPRDGERLPAVVYYHGGGFVLGSVETHDHVCRRLANLSGAVVVSVDYRLAPEHKFPAAVEDAYDAAKWVADNYDKLGVDNGKIAVAGDSAGGNLAAVTAIMARDRGESFVKYQVLIYPAVNLTGSPTVSRVEYSGPEYVILTADLMAWFGRQYFSKPQDALSPYASPIFADLSNLPPALVITAEYDPLRDEGELYAHLLKTRGVRAVAVRYNGVIHGFVNFYPILEEGREAVSQIAASIKSMAVA
Yuji Hotta et al. (2002)
β Extremely Stable and Versatile Carboxylesterase from a Hyperthermophilic Archaeon
Applied and Environmental Microbiology
Β· doi:10.1128/AEM.68.8.3925-3931.2002 β
Β· Activity - Classical Stability - Incubation
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Database ID
UniProt: Q8NKS0 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (18 measurements)
18 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethylformamide (DMF) | 50% | 4053 | 296 | U/mg | 20 mM Tris-HCl | 8 | 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in U/mg) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 50% | 4053 | 2148 | U/mg | 20 mM Tris-HCl | 8 | 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in U/mg) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Acetonitrile | 50% | 4053 | 119 | U/mg | 20 mM Tris-HCl | 8 | 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in U/mg) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 50% | 4053 | 42 | U/mg | 20 mM Tris-HCl | 8 | 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in U/mg) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 50% | 4053 | 101 | U/mg | 20 mM Tris-HCl | 8 | 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in U/mg) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 50% | 4053 | 556 | U/mg | 20 mM Tris-HCl | 8 | 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Classical aqueous control (in U/mg) |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 80% | 100 | 116 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 80% | 100 | 96 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 80% | 100 | 90 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 50% | 100 | 109 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 80% | 100 | 94 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 80% | 100 | 103 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Methanol | 80% | 100 | 89 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethylformamide (DMF) | 50% | 100 | 124 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100 | 101 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Acetonitrile | 50% | 100 | 117 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Isopropanol | 50% | 100 | 110 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase | Ethanol | 50% | 100 | 105 | % | Incubation: 20 mM Tris-HCl buffer, Assay: 20 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 70Β°C | 1 mM p-Nitrophenyl hexanoate | Hexanoic Acid , p-Nitrophenol | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.