Lipase (gene lip2) from Psychrobacter SC65A.3

Enzyme Description

Species
Psychrobacter SC65A.3 β†—
Extremophile
Yes "psychrophilic " organism
EC Number
3.1.1.3 β†—

Sequence

Length: 483 amino acids
MSNSTVLSVNTLLNKAVKTLNLMSFGQDKNPKSTDINLSDEIIDIEESALQDSREDKGLSIKEKILEHHLMTNYQPHLLHYAIKSFGCLPTPILESLIKCLDGPTSKQYLHVDAHLRLILAVNSKLKTPLQLIEMSELRKRFATDAVAMQAPKVWQQASDNLLSNLKQFHKKGDSAISWQDRTITNADDGDMTIRCYQNETSDNGFGFKKEQTSNPDETVLLFFHGGGFCIGDLNTHHEFCHAICEQTGWPVISVDYRLAPEHPAPAAVRDCISAYAWLAEHCEEFGALPSRIVLAGDSAGGGLSTLMAQQIITPNKEAWLDLGDEGQKTFDILQGLPHPMAQMPLYPVTDIETDYPSWELYGEGLLLDHADVAIFDAACLENSPLPRQHILTSPMLGDNRQVCPSYVVAAELDVLRDEAFAYANQLKSFGIAVQTHTVLGAPHGFIHFMSVHQRLGQETQHIITGFANFVREIIKTRALLSA
Victoria I. Paun et al. (2024) β€” Cold-Active Lipase from the Ice Cave Psychrobacter SC65A.3 Strain, a Promising Biocatalyst for Silybin Acylation
Molecules  Β· doi:10.3390/molecules29215125 β†—  Β· Activity - Classical
16 measurements ▼
Database ID
NCBI: WP_263241157.1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Ethanol 1% 100.0 55.2 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Ethanol 5% 100.0 43.9 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Ethanol 10% 100.0 42.3 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Ethanol 15% 100.0 38.5 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Ethanol 20% 100.0 8.9 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 100.0 80.3 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 73.5 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 57.4 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethylformamide (DMF) 1% 100.0 73.3 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethylformamide (DMF) 5% 100.0 49.6 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethylformamide (DMF) 10% 100.0 42.0 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Dimethylformamide (DMF) 20% 100.0 37.2 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Tetrahydrofuran (THF) 2% 100.0 73.0 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Tetrahydrofuran (THF) 5% 100.0 48.8 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Tetrahydrofuran (THF) 15% 100.0 43.1 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 347 nm) in the presence of organic solvent Tetrahydrofuran (THF) 20% 100.0 19.4 % 50 mM Tris-HCl buffer 7.2 25Β°C 3.75 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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