Poly(3-hydroxybutyrate) depolymerase from Purification and character
Enzyme Description
Sequence
MQLLSGRQWRWLVGFFAALATTSVLAAVTPGPGTWSAQQTWAADSVNGGNLTGYYYWPASQPTTPNGQRALILVLHGCLQTASGDVIDSSSAAGFNWKAIADQYGAIILAPNATGNVYSNHCWDYANTSPSRSAGHVGVLLDLVNRFVSNSQYAIDPNQVYVTGLSSGGGMTMVLGCIAPDVFAGIGINAGPPPGTTTSQISYVPSGYTATTAANQCKAWAGSNAGKFATQIAGAVWGTSDYTVAQAYGPLDTAAFRILYGGTFSQGTKVTIPGGGTNTPYSDSSGKVRTHEISVTGMSHAWPAGSGGKNSNYVDATHINYPVFLMDYWVKNNLRSVSGPTQSGSAPTGLAVAGTTQTSISLSWNAVAGASSYNVYRNGSKVGSSVSASYTDAGLIAGTTYSYSVSEIDPSAGESAQSSSVSATTQSSFACTETTATNYAHVQAGRAHDSSGTAYANGSNQSMGLDNVFYSNTLAQTSAGYYVIGIGP
Nor Azura Azami et al. (2019)
β Purification and characterization of new bio-plastic degrading enzyme from Burkholderia cepacia DP1
Protein Expression and Purification
Β· doi:10.1016/j.pep.2018.10.008 β
Β· Activity - Classical
▼
Database ID
β
Sequence Annotation
Explicit - Provided
Protein Source
Purified, bacterium Burkholderia cepacia DP1
Experimental Data (3 measurements)
3 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (solution turbidity absorbance measurement, 650 nm) | Ethanol | 10% | 100 | 100 | % | 100 mM phosphate buffer | 6 | 45Β°C | 250 W), P(3HB) polymer granules, sonicated (60 kHz | 3-hydroxybutyrate (3HB) monomers , short-chain oligomers | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (solution turbidity absorbance measurement, 650 nm) | Methanol | 10% | 100 | 95 | % | 100 mM phosphate buffer | 6 | 45Β°C | 250 W), P(3HB) polymer granules, sonicated (60 kHz | 3-hydroxybutyrate (3HB) monomers , short-chain oligomers | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (solution turbidity absorbance measurement, 650 nm) | Acetone | 10% | 100 | 100 | % | 100 mM phosphate buffer | 6 | 45Β°C | 250 W), P(3HB) polymer granules, sonicated (60 kHz | 3-hydroxybutyrate (3HB) monomers , short-chain oligomers | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.