| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
15% |
100.0 |
β |
80.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
β |
51.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
40% |
100.0 |
β |
18.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
15% |
100.0 |
β |
56.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
25% |
100.0 |
β |
37.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
40% |
100.0 |
β |
9.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
15% |
100.0 |
β |
83.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
25% |
100.0 |
β |
63.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
40% |
100.0 |
β |
45.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
15% |
100.0 |
β |
115.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
25% |
100.0 |
β |
119.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
40% |
100.0 |
β |
94.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
15% |
100.0 |
β |
104.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
25% |
100.0 |
β |
76.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
40% |
100.0 |
β |
19.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
15% |
100.0 |
β |
119.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| WT |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
25% |
100.0 |
β |
78.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| I2G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62G |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
5.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62A |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62V |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62I |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62L |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
5.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62M |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62F |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
35.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62W |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61E |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60R |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62H |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
35.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62K |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
35.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62S |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62T |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62N |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62Q |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A3C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G10C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S12C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
15.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| P34C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I2P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q4P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I5P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H6P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I7P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L8P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E9P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R11P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D13P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E14P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| Q15P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K16P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E17P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T18P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L19P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I20P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R21P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E22P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V23P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S24P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E25P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A26P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I27P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S28P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R29P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L31P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| D32P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A33P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L35P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T36P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S37P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V38P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R39P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V40P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I41P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I42P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| T43P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E44P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| M45P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A46P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K47P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G48P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| H49P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| F50P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G51P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| I52P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G53P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| G54P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| E55P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
0.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| L56P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| A57P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S58P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| K59P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| V60P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R61P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| R62P |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
50.0 |
50.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 0.5 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
500 rpm for 60 first seconds |
Classical aqueous control (in %) in 5% ethanol | Categorical data/deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
15% |
100.0 |
80.0 |
92.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
51.0 |
65.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
40% |
100.0 |
18.0 |
21.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
15% |
100.0 |
80.0 |
102.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
51.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
40% |
100.0 |
18.0 |
45.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
15% |
100.0 |
80.0 |
106.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
25% |
100.0 |
51.0 |
75.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Ethanol |
40% |
100.0 |
18.0 |
44.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% ethanol | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
15% |
100.0 |
56.0 |
70.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
25% |
100.0 |
37.0 |
52.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
40% |
100.0 |
9.0 |
37.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
15% |
100.0 |
56.0 |
77.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
25% |
100.0 |
37.0 |
58.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
40% |
100.0 |
9.0 |
25.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
15% |
100.0 |
56.0 |
59.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
25% |
100.0 |
37.0 |
52.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Methanol |
40% |
100.0 |
9.0 |
13.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
15% |
100.0 |
83.0 |
94.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
25% |
100.0 |
63.0 |
84.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
40% |
100.0 |
45.0 |
51.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
15% |
100.0 |
83.0 |
92.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
25% |
100.0 |
63.0 |
85.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
40% |
100.0 |
45.0 |
54.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
15% |
100.0 |
83.0 |
100.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
25% |
100.0 |
63.0 |
87.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
1,3-Propanediol |
40% |
100.0 |
45.0 |
44.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
15% |
100.0 |
115.0 |
146.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
25% |
100.0 |
119.0 |
198.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
40% |
100.0 |
94.0 |
178.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
15% |
100.0 |
115.0 |
110.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
25% |
100.0 |
119.0 |
133.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
40% |
100.0 |
94.0 |
118.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
15% |
100.0 |
115.0 |
126.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
25% |
100.0 |
119.0 |
164.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Dimethyl Sulfoxide (DMSO) |
40% |
100.0 |
94.0 |
188.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
15% |
100.0 |
104.0 |
140.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
25% |
100.0 |
76.0 |
132.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
40% |
100.0 |
19.0 |
11.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
15% |
100.0 |
104.0 |
115.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
25% |
100.0 |
76.0 |
94.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
40% |
100.0 |
19.0 |
44.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
15% |
100.0 |
104.0 |
123.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
25% |
100.0 |
76.0 |
110.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
Isopropanol |
40% |
100.0 |
19.0 |
3.5 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
15% |
100.0 |
119.0 |
68.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30C |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
25% |
100.0 |
78.0 |
52.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
15% |
100.0 |
119.0 |
103.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| S30Y |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
25% |
100.0 |
78.0 |
62.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
15% |
100.0 |
119.0 |
114.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |
| A33D |
Activity - Classical |
Activity of cell free extracts measured by absorbance spectrophotometry (decrease in Ξ²-Nitrostyrene absorbance measurement, 320 nm) in the presence of organic solvent |
tert-Butanol |
25% |
100.0 |
78.0 |
18.0 |
% |
20 mM sodium phosphate buffer |
7.3 |
Room temperature |
50 mM Acetaldehyde, 2 mM Ξ²-Nitrostyrene |
(R)-4-Nitro-3-phenylButyraldehyde |
β |
β |
Classical aqueous control (in %) in 5% organic solvent | Deducted temperature |