M42 aminopeptidase (gene APHap) from Haladaptatus litoreus

Enzyme Description

Species

Haladaptatus litoreus ↗

Extremophile

Yes "halophilic" organism

EC Number

3.4.11.1 ↗

Sequence

Length: 355 amino acids

MHERHREFLQTLLTTPSPSGFEVPGQRAWVEYVSEFADEVRTDEYGNAVAVVEGDGPEIAFAGHADQIGLIVAGIDEDGFIRVDKIGGSDRTVTKGQQVDIHTDDGVVNGVVGQRAIHLRDPADDEFEDITEQHVDIGASSEAEAKELVDVGDPIVFTPNIEELHGTRLAGPGIDNRVGTWVAAEALRRAADKGADATVYAVSTVQEEVGLQGAKMVGFDLDPDAIVAVDVTHAMDSPDVTNEEQFKDIDIDLGGGPVVARGSTNHIEVVKAIREAAKNADIPVQLQAAGIRTGTDADAFYTASGGTPSLNLGLPNRYMHTPVEVIDTDDLELSADLLAEFAVRASDRDGFSVDL

Jing Hou et al. (2025) — A Halophilic Aminopeptidase With Broad Substrate Specificity: Exploring Its Catalytic Potential and Application in Salt-Fermented Foods

Journal of Food Science · doi:10.1111/1750-3841.70218 ↗ · Activity - Classical

7 measurements ▼

Database ID

UniParc: UPI0009703422 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host archaeon Haloferax volcanii H1424

Experimental Data (7 measurements)

7 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100	117	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100	105	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	103	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	78	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	15%	100	78	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Glycerol	15%	100	63	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	15%	100	48	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine , p-Nitroaniline	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	15%	100	117	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	15%	100	105	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	103	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	15%	100	78	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	15%	100	78	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Glycerol	15%	100	63	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitroaniline absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	15%	100	48	%	20 mM Tris-HCl buffer, 2 M NaCl	8	50°C	1 mM Leucine p-nitroanilide	L-Leucine, p-Nitroaniline	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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