Chitinase A (gene ChiA) from Bacillus proteolyticus

Enzyme Description

Species
Bacillus proteolyticus β†—
Extremophile
No
EC Number
3.2.1.14 β†—

Sequence

Length: 676 amino acids
MAMRSQKFTLLLLSLLLFLPLFLTNFITPNLALADSPKQSQKIVGYFPSWGVYGRNYQVADIDASKLTHLNYAFADICWNGKHGNPSTHPDNPNKQTWNCKESGVPLQNKEVPNGTLVLGEPWADVTKSYPGSGTTWEDCDKYARCGNFGELKRLKAKYPHLKTIISVGGWTWSNRFSDMAADEKTRKVFAESTVSFLRAYGFDGVDLDWEYPGVETIPGGSYRPEDKQNFTLLLQDVRNALNKAGAEDGKQYLLTIASGASQRYADHTELKKISQILDWINIMTYDFHGGWEATSNHNAALYKDPNDPAANTNFYVDGAIDVYTNEGVPVDKLVLGVPFYGRGWKSCGKENNGQYQPCKPGSDGKLASKGTWDDYSTGDTGVYDYGDLAANYVNKNGFVRYWNDTAKVPYLYNATTGTFISYDDNESMKYKTDYIKTKGLSGAMFWELSGDCRTSPKYSCSGPKLLDTLVKELLGGPISQKDTEPPTNVKNIVVTNKNSSSVQLSWTASTDNVGVTEYEITAGEEKWSSTTNSITIKNLKPNTEYTFSVIAKDASGNKSQPTALTVKTDEANTTPPDGNGTATFSVTSNWGSGYNFSIIIKNNGTTPIKNWKLEFDYSGNLTQVWDSKINSKTNNHYVITNAGWNGEIPPGGSITIGGAGTGNPAELLNAVISEN
Fang Liu et al. (2025) β€” Preparation and enzymatic activity analysis of rChiA-DP derived from the Bacillus proteolyticus IMH/B-1 Strain isolated from Dermacentor nuttalli
Scientific Reports  Β· doi:10.1038/s41598-025-17223-7 β†—  Β· Activity - Classical
14 measurements ▼
Database ID
UniProt: A0A059V772 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Methanol 10% 100.0 119.2 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Methanol 20% 100.0 56.9 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Ethanol 10% 100.0 109.2 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Ethanol 20% 100.0 60.6 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Glycerol 10% 100.0 100.3 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Glycerol 20% 100.0 44.7 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Acetic Acid 10% 100.0 18.1 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Acetic Acid 20% 100.0 15.6 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 88.0 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 40.0 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Isopropanol 10% 100.0 120.0 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Isopropanol 20% 100.0 47.5 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Isoamyl Alcohol 10% 100.0 120.3 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (colorimetric assay, reaction product of N-acetyl-D-glucosamine and p-dimethylaminoBenzaldehyde absorbance measurement, 585 nm) in the presence of organic solvent Isoamyl Alcohol 20% 100.0 30.8 % 200 mM disodium hydrogen phosphate buffer 7 40Β°C ? mM Colloidal Chitin N-Acetyl-D-Glucosamine β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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