Laccase (gene rLac-HhC125) from Halalkalibacterium halodurans C‐125

Enzyme Description

Species
Halalkalibacterium halodurans C‐125 β†—
Extremophile
Yes "halophilic" organism
EC Number
1.10.3.2 β†—

Sequence

Length: 500 amino acids
MKKSYGVMGGVLAVFFAIAGCSSDDHSEMDHGNNQQDEQNEGTFEDGKKVFNLEVTETHWMFNDEVMMDAWTYNGTLPGQEIRVQEGDEVVINVKNSLNVPTALHLHGFPVPNEMDGVPGVTQNAIMPGEEFTYEYQANVPGTYWYHSHQDGATQVDRGLYGVFIVEPVDPESYDTDVAIVIDEFASMGMGMGMDHSTMDHGDMEHDGNDDMSHSEMMNEMYDTMVINGKADPQIESINVVEGNRVKLRFVNAGLFTQVVSIPEHSFKITHYDGQPVNEPDVLQDTAFRIAPAERYDLEIIMDNPGAWGIQVFAEENQERLQALIPLVYEEYEDEELQTVDSISSFFDLTTYGIPKELDIGDITKEYDMLLGSDDGGDTFTINGKKFPNHEIYDVEEGDMVKFTIINDTDFDHPMHLHGDFFHVISKGGKAIQGSPVLKDTLNVRPGETYEIVFEAKNPGNWMFHCHEFHHASGGMVAEIHYEGFELPFTPDPNIPNMPE
Jihene Maati et al. (2024) β€” Biochemical characterization of a recombinant laccase from Halalkalibacterium halodurans C-125 and its application in the biotransformation of organic compounds
Biotechnology Letters  Β· doi:10.1007/s10529-024-03532-w β†—  Β· Activity - Classical Activity + Stability - Incubation
8 measurements ▼
Database ID
NCBI: BAB05801.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Methanol 10% 100.0 101.3 % 100 mM Tris-HCl buffer 8 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Ethanol 10% 100.0 110.7 % 100 mM Tris-HCl buffer 8 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 84.1 % 100 mM Tris-HCl buffer 8 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Acetonitrile 10% 100.0 177.2 % 100 mM Tris-HCl buffer 8 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Classical aqueous control (in %)
Activity + Stability - Incubation Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Methanol 10% 100.0 94.6 % Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Ethanol 10% 100.0 107.5 % Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 91.3 % Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent
Activity + Stability - Incubation Activity after incubation (20 minutes at 50Β°C), measured by absorbance spectrophotometry (oxidized Syringaldazine absorbance measurement, 530 nm) in the presence of organic solvent Acetonitrile 10% 100.0 201.1 % Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 8, Assay: 8 Incubation: 50Β°C, Assay: 50Β°C 0.025 mM Syringaldazine Oxidized Syringaldazine 2 mM CuSOβ‚„ β€” Non-incubated control (in %), assay in the presence of organic solvent | Uncertainty about whether assay really was in the presence of organic solvent

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Activity + Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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