Alginate lyase (gene Alg0392) from Alteromonas sp. A1‐6

Enzyme Description

Species
Alteromonas sp. A1‐6 β†—
Extremophile
No
EC Number
4.2.2.3 β†—

Sequence

Length: 737 amino acids
MLAIGISMSSLPIQANNHPNLILTEHGVERMRDNLGQVPLFDKSLAVIKQEVDAEIELGIDTPIPKDFSGGYTHQRHKQNFFIAQKAGVLYQILEDEKYARYIRDMLFQYEAMYKDLPLHPQERSYARGKLFWQCLNDSNWLVYMSQAYDAIYDYLSAQERQTLEDNLFRPFADFISLENPQFFNRVHNHSTWGIAAVGMIGLVMNDEELIQRALYGIDDDGLEVGGTDNDGGTIKVEGQRKGFFANLEEPFSPDGYYTEGPYYQRYAMYPFLILALAMENNRPDLKVLQHKDNVLLKGIDALLNLSDADGEFFPLNDAQKGMSYKKASLVTAVDLAYFYGEKDPRLLSVVEKQGEVLLQESGLEAAMALRDGKAEPFVKSSVNLSDGPDGDQGGVGILRYGDEELTLVFKYAAQGLSHGHYDKLSFSLYERGEEVLQDYGLARFVNIGQKGGGNYLKENTTWAKTTIAHNTLSVNGKKHFDGKYQVASQHHSELFFFDAEKDNVKVVSATEENAYPGIFMHRTMAMIKDENFENPFVLDILRVKSDEDNQYDLPFYYMGQVIDKNFTYQSPESLSPFGEDNGYQHLFVEGFGKANPGNTKFQWLGSGKYYTLTTTTRAEDEIYLTRIGANDPDFNLRRDAAITLRRNDTANTVFVSIVEPHGSYSPVNESSINSRSSIEKLTVVLDTEDYTGASITDVKGDTQLFIVSNNNNSNLAKHTVTIEGNTYSWSGPYYYQ
Tong Liang et al. (2025) β€” Characterization of a novel alginate lyase Alg0392 with organic solvent-tolerance from Alteromonas sp. A1-6
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-025-13506-1 β†—  Β· Activity - Classical
8 measurements ▼
Database ID
UniParc: UPI003D37E8F0 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 5% 100.0 80.2 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 20% 100.0 75.8 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 5% 100.0 74.6 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 20% 100.0 50.9 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 5% 100.0 85.0 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 20% 100.0 46.8 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 82.2 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 71.6 % 50 mM sodium phosphate buffer 6 30Β°C 4 mg/ml Alginate Reducing sugars β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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