Catechol 2,3-dioxygenase from a coil-contaminated soil metagenomic library

Enzyme Description

Species
Na (coal-cotaminated soil metagenomic library)
Extremophile
No
EC Number
1.13.11.2 β†—

Sequence

Length: 304 amino acids
MGEVVGVGLIAHVPTIMLPKEIRMELNEGKDTTLVPSLEQLRTDVFDTLDYDTVIVLDSHWASTVEFIVTAQERRQGLFTSEELPRGMHGVPFDYRGDPELARLIGEQDEKNGTWITPFDDPDLPVMYATINLWKYLGEGLPDKRWISVSVCQTATDEDFLRCGRAIGEAIAKSDRKVILLASGALSHTFFKLRELRKHEMADPAHIFSDAAREADYERLEWFKAGDHAKVLETMPAFHKVRPEAMFGHWLMTIGAIGEEACTAPGIQLSDYENSIGTGQVHVWFPQPEGGFPRPKDLVLSRED
Yi-Xiang Wang et al. (2025) β€” Molecular cloning, characterization, and structural stability analysis of a rare acidic catechol 2,3-dioxygenase from the metagenome of coal-polluted soil
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2025.143652 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
UniParc: UPI003A60A512 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Methanol 1% 100.0 116.7 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Methanol 5% 100.0 108.3 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Methanol 15% 100.0 97.8 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Ethanol 1% 100.0 121.7 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Ethanol 5% 100.0 127.6 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Ethanol 15% 100.0 110.2 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Isopropanol 1% 100.0 117.9 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Isopropanol 5% 100.0 123.6 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Isopropanol 15% 100.0 119.5 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Acetaldehyde 1% 100.0 119.5 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Acetaldehyde 5% 100.0 142.9 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Acetaldehyde 15% 100.0 121.9 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 100.0 102.3 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 105.2 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (2-hydroxymuconic semialdehyde absorbance measurement, 375 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15% 100.0 106.7 % 100 mM phosphate buffer 4 55Β°C 0.5 mM Catechol 2-hydroxymuconic semialdehyde β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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