Serine protease (gene aprBP) from Bacillus patagoniensis DB-5
Enzyme Description
Sequence
AEEAKEKYLIGFTEQEAVSTFVEQIEEEEVSISEVDDVEIDLLYEFETIPVLSVELNPEDVASLESDPAISYIEEDAEVTTMAQSVPWGISRVQAQSAHNRGITGSGVKVAVLDTGISTHEDLNVRGGASFVAGEPGYQDGNGHGTHVAGTIAALNNSIGVLGVAPNAELYAVKVLGASGSGSISGIAQGLQWAGNNGMHIANMSLGTSAPSATLEQAVNAATAQGVLVIAASGNSGAGSVGYPARYANAMAVGATDQNNNRASFSQYGAGLDIVAPGVGVQSTYPGNRYASLNGTSMATPHVAGVAALVKQKNPSWSNVQVRNHLKNTATNLGNTNLYGSGLVNAEAATR
Zhimin Li et al. (2025)
β Redox and solvent-stable alkaline serine protease from Bacillus patagoniensis DB-5: heterologous expression, properties, and biotechnological applications
Frontiers in Microbiology
Β· doi:10.3389/fmicb.2025.1558419 β
Β· Stability - Incubation
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Database ID
NCBI: XUA96904.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | Methanol | 50% | 100.0 | 335.66 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | Acetone | 50% | 100.0 | 400.63 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | Glycerol | 50% | 100.0 | 374.52 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | Dimethyl Sulfoxide (DMSO) | 50% | 100.0 | 245.85 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | n-Hexane | 50% | 100.0 | 254.14 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | Ethyl Acetate | 50% | 100.0 | 445.85 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
| Stability - Incubation | Activity after incubation (72 hours at 25Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement) in aqueous phase | Chloroform | 50% | 100.0 | 5.73 | % | ? | ? | Incubation: 25Β°C, Assay: ? | ? mM Casein | free amino acids , Peptides | β | Incubation: 150 rpm | Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.