Ξ²-mannase (gene ManEM6) from Hermetia illucens gut metagenomic library

Enzyme Description

Species
Na (Hermetia illucens gut metagenomic library)
Extremophile
No
EC Number
3.2.1.78 β†—

Sequence

Length: 394 amino acids
MMHKTITFIFCFLWLSVAYASDFLLVDPVATAETKALFYNLKKIEQSDRVIFGQQDATLYGRSWVGDSNRSDVKDLCGQHPALIGFDFEAATESDPVKFENIKNRLVKATKEVYRQGGIITFSWHSRNPANDGSFYWEKNPVESVKDILPEGKLHAKYKQYLESIAKVINDCKGDNGELIPIIFRPFHEFDGDWFWWGKGHCSKDEFIALWQFTVTYLRDDMEVHNLLYAFSPDCKFFTEEEFMEYYPGDKYVDIIGMDNYWDFRPDGANDLQLAAQKLKIVSDIATKKNKIAALTETGLEGVSNPLWYTQVLLPVLKGTKIAYVMVWRNASDLPTHYYTPTVGHPAAQDFIRFSEDKNILFQSDLPDMYKSSYKLQDSGVIRGSGSALPVIKQ
Dong-Gwan Kim et al. (2024) β€” Isolation of a Novel Low-Temperature-Active and Organic-Solvent-Stable Mannanase from the Intestinal Metagenome of Hermetia illucens
International Journal of Molecular Sciences  Β· doi:10.3390/ijms26010216 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
UniProt: A0A1W6DZE9 β†—
Sequence Annotation
Explicit- Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100.0 97.0 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Dimethylformamide (DMF) 10% 100.0 95.1 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Methanol 10% 100.0 97.9 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetonitrile 10% 100.0 80.2 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Ethanol 10% 100.0 97.6 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Acetone 10% 100.0 98.9 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Isopropanol 10% 100.0 62.9 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Pentanol 10% 100.0 29.9 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Hexanol 10% 100.0 30.5 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Chloroform 10% 100.0 6.8 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Benzene 10% 100.0 28.1 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase 1-Heptanol 10% 100.0 29.4 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Toluene 10% 100.0 30.3 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase Cyclohexane 10% 100.0 31.6 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at 30Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase n-Hexane 10% 100.0 25.0 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: 30Β°C, Assay: 40Β°C 0.5% (w/v) Locust bean gum Reducing sugars β€” β€” Water-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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