Ξ±-amylase (Taka-amylase) from Aspergillus oryzae
Enzyme Description
Sequence
ATPADWRSQSIYFLLTDRFARTDGSTTATCNTADQKYCGGTWQGIIDKLDYIQGMGFTAIWITPVTAQLPQTTAYGDAYHGYWQQDIYSLNENYGTADDLKALSSALHERGMYLMVDVVANHMGYDGAGSSVDYSVFKPFSSQDYFHPFCFIQNYEDQTQVEDCWLGDNTVSLPDLDTTKDVVKNEWYDWVGSLVSNYSIDGLRIDTVKHVQKDFWPGYNKAAGVYCIGEVLDGDPAYTCPYQNVMDGVLNYPIYYPLLNAFKSTSGSMDDLYNMINTVKSDCPDSTLLGTFVENHDNPRFASYTNDIALAKNVAAFIILNDGIPIIYAGQEQHYAGGNDPANREATWLSGYPTDSELYKLIASANAIRNYAISKDTGFVTYKNWPIYKDDTTIAMRKGTDGSQIVTILSNKGASGDSYTLSLSGAGYTAGQQLTEVIGCTTVTVGSDGNVPVPMAGGLPRVLYPTEKLAGSKICSSS
Khomaini Hasan et al. (2024)
β Enzymatic Performance of Aspergillus oryzae Ξ±-Amylase in the Presence of Organic Solvents: Activity, Stability, and Bioinformatic Studies
Bioinformatics and Biology Insights
Β· doi:10.1177/11779322241234767 β
Β· Activity - Classical
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Database ID
PDB: 7TAA β
Sequence Annotation
Inferred - sequence of PDB given by authors and used for MD simulations
Protein Source
Commercially purchased
Experimental Data (20 measurements)
20 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | 1-Butanol | 25% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Diethyl Ether | 30% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Diethyl Ether | 25% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Diethyl Ether | 20% | 100.0 | 20.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Diethyl Ether | 15% | 100.0 | 14.88 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Acetone | 30% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Acetone | 25% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Acetone | 20% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Acetone | 15% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | 1-Butanol | 30% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Methanol | 15% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | 1-Butanol | 20% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | 1-Butanol | 15% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Ethanol | 30% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Ethanol | 25% | 100.0 | 18.48 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Ethanol | 20% | 100.0 | 29.25 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Ethanol | 15% | 100.0 | 15.2 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Methanol | 30% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Methanol | 25% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (colorimetric assay, starch-iodine complex absorbance decrease measurement, 600 nm) in the presence of organic solvent | Methanol | 20% | 100.0 | 0.0 | % | ? | ? | 50Β°C | 0.2% Starch | Reducing sugars | β | β | Classical aqueous control (in %) | Uncertainty about protein sequence (rom PDB given by authors and used for MD simulations) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.