GH10 endo-xylanase (gene Scxyn5) from Schizophyllum commune DB01

Enzyme Description

Species
Schizophyllum commune DB01 β†—
Extremophile
No
EC Number
3.2.1.8 β†—

Sequence

Length: 309 amino acids
AAGLKYLGSATDNPELTDTDYLAILSDSDEFGQLTPGNSMKWDATEPTQGQFSFDNADAIVELAQNNSQLIRGHTCVWYSQLPSWVSNGSWDADSLNEAMTTHTSTVVDHFRGKIYSWDVVNEAFEDDGTFRQNVFYTTIGEDYIANAFKAAREADPDAKLYINDYNIEGTGAKADALYTFVTSLLNAFVPIDGIGMQAHLIVGSVPTTIQENIARFTALGLEVALTELDIRMPVPAAETDLEQQKADYESVVGACAAVDGCVGVTVWDYTDKYSWVPSVFDGYGAALPWDENLEKKPAYDGIVSGLGA
Yuchun Liu et al. (2024) β€” Biochemical characterization of an organic solvent- and salt-tolerant xylanase and its application of arabinoxylan-oligosaccharides production from corn fiber gum
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2024.136146 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided (but 61 first residues missing)
Protein Source
Recombinant, host yeast Pichia pastoris GS115

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 2% 100.0 118.1 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 2% 100.0 141.4 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 2% 100.0 137.46 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Butanol 2% 100.0 2.69 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 2% 100.0 167.47 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 5% 100.0 57.75 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 5% 100.0 52.14 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 5% 100.0 84.49 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Butanol 5% 100.0 0.0 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 105.08 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 10% 100.0 36.34 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 10% 100.0 44.98 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 10% 100.0 46.52 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Butanol 10% 100.0 0.0 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 83.05 % 100 mM citrate buffer 6 70Β°C 0.5% (w/v) Soluble birchwood xylan Reducing sugars β€” β€” Classical aqueous control (in %) | 61 first residues from protein sequence missing

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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