GH5 endoglucanase from Bacillus sp. G131

Enzyme Description

Species
Bacillus sp. G131 β†—
Extremophile
No but "thermostable" enzyme
EC Number
3.2.1.4 β†—

Sequence

Length: 461 amino acids
ASAASQTPVAVNGQLTLKGTQLVNQKGKAVQLKGISSHGLQWYGDYVNKDSKLWLRDDWGINVFRAAMYTGEGGYIDNPSVKNKVKEAVEAAKELGIYVIIDWHILSDGNPNQNKAKAKEFFNEMSRLYGKTPNVIFEIANEPNGDNVWNNDIKPYAEDILSVIRKNSPKNIVIVGTGTWSQDVNDAADNQLKDGNVMYALHFYAGTHGQSLRDKADYALSKGAPIFVTEWGTSDASGNGGVYLDQSREWLKYLDSKKISWVNWSLCDKQESSAALNSGASKKGGWSQSDlSSSGKFVRENIRSGSNGSSGDSGSDSKGSDQKDQKKDQDKPGQDSGAAANTIAVQYRAGDNNVNGNQIRPQLNIKNNSKKTVSLNRITVRYWYKTNHKGQNFDCDYAQIGCSKLTHKFVQLKKAVNGADTYLEVGFKNGTLAPGASTGEIQIRLHNDNWSNYAQIGDYSF
Nazanin Gholampour-Faroji et al. (2024) β€” Characterization, structural, and evolutionary analysis of an extremophilic GH5 endoglucanase from Bacillus sp. G131: Insights from ancestral sequence reconstruction
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2024.134311 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
NCBI: XCG98628.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number (first 26 residues from GenBank sequence absent from the mature protein)
Protein Source
Purified, bacterium heat-adapted extremophileBacillus sp. G131

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Dimethyl Sulfoxide (DMSO) 10% 100.0 65.09 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Methanol 10% 100.0 61.67 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Ethanol 10% 100.0 67.86 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Acetone 10% 100.0 61.97 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Isopropanol 10% 100.0 70.72 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Dimethyl Sulfoxide (DMSO) 20% 100.0 54.05 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Methanol 20% 100.0 48.17 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Ethanol 20% 100.0 57.2 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Acetone 20% 100.0 52.35 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Isopropanol 20% 100.0 49.54 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Dimethyl Sulfoxide (DMSO) 30% 100.0 44.06 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Methanol 30% 100.0 37.79 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Ethanol 30% 100.0 43.84 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Acetone 30% 100.0 37.83 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (4 hours at 25Β°C), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous conditions Isopropanol 30% 100.0 39.86 % ? ? Incubation: 25Β°C, Assay: 50Β°C ? mM CMC (Carboxymethylcellulose sodium) Reducing sugars β€” Incubation: 120 rpm Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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