Glutamate dehydrogenase from Aeropyrum pernix K1

Enzyme Description

Species
Aeropyrum pernix K1 β†—
Extremophile
Yes "hyperthermophilic" organism
EC Number
1.4.1.2 β†—

Sequence

Length: 418 amino acids
MQPTDPLEEARAQLRRAVDLLGYDDYVYEVLANPDRVLQVRVTIKMDDGTVKTFLGWRSQHNSALGPYKGGVRYHPNVTMNEVIALSMWMTWKNSLAGLPYGGGKGGVRVNPKILSPRELELLSRKYFESISDIVGVDQDIPAPDVYTDPQVMSWFLDEYNRVKRGQFFGVVTGKPVELGGLNARIVSTGYGVAVSTRVAAEKFLGGLEGRTVAVQGYGNVGYYAAKFLAEMGAKIVAVSDSRGGIYDPEGIDPEEALKVKRSTGTVANYQRGKKISTMEILELPVDILVPAAIEEVITDENADRIKAKIISEGANGPTTTAAEKILVKKGVLVLPDILANAGGVIMSHIEWVNNRMGGWITDEEALKKLEQKMVENTKTVITYWEKNLKPEENSLRDAAYMIAVERVFRAMKLRGWI
I. Helianti et al. (2001) β€” Characterization of native glutamate dehydrogenase from an aerobic hyperthermophilic archaeon Aeropyrum pernix K1
Applied Microbiology and Biotechnology  Β· doi:10.1007/s002530100575 β†—  Β· Activity - Classical
14 measurements ▼
Database ID
UniProt: Q9YC65 β†—
Sequence Annotation
Inferred - from protein name
Protein Source
Purified, archaeon Aeropyrum pernix K1

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 10% 100 160 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 25% 100 284 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 40% 100 287 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 50% 100 167 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 60% 100 101 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 10% 100 147 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 25% 100 333 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 40% 100 359 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 10% 100 161 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 25% 100 255 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Acetonitrile 40% 100 318 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100 128 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 25% 100 208 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do
Activity - Classical Activity measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40% 100 255 % 100 mM sodium phosphate 7 50Β°C 20 mM Monosodium L-glutamic acid NH4+ , Ξ±-ketoglutarate 0.1 mM NADP β€” Classical aqueous control (in %) | To do

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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