Endochitinase (gene Chit36‐TA) from Trichoderma asperellum

Enzyme Description

Species

Extremophile

EC Number

3.2.1.14 ↗

Sequence

Length: 400 amino acids

MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSASIAAKEEGVSLEKREAEAQNATCALKGKPAGKVLMGYWENWDGAANGVHPGFGWTPIENPIIKQNGYNVINAAFPVILSDGTALWENDMAPDTQVATPAEMCEAKAAGATILLSIGGATAGIDLSSSAVADKFIATIVPILKQYNFDGIDIDIETGLTNSGNINTLSTSQTNLIRIIDGVLAQMPSNFGLTMAPETAYVTGGSITYGSIWGAYLPIIQKYVQNGRLWWLNMQYYNGDMYGCSGDSYAAGTVQGFIAQTDCLNAGLTIQGTTIKVPYDMQVPGLPAQSGAGGGYMNPSLVGQAWDHYNGALKGLMTWSINWDGAGNWTFGDNLLTRIGHHHHHH

Luisa Gebele et al. (2024) — Recombinant expression and characterization of the endochitinase Chit36-TA from Trichoderma asperellum in Komagataella phaffii for chitin degradation of black soldier fly exuviae

Bioprocess and Biosystems Engineering · doi:10.1007/s00449-024-03067-4 ↗ · Activity - Classical

4 measurements ▼

Database ID

UniParc: UPI002B286993 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host yeast Komagataella phaffii

Experimental Data (4 measurements)

4 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	91	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Ethanol	10%	100	85	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Acetone	10%	100	85	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	87	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents

Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	91	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Ethanol	10%	100	85	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Acetone	10%	100	85	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents
Activity - Classical	Activity measured by fluorescence spectroscopy (4-MU fluorescence measurement, excitation 365 nm, emission 445 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	100	87	%	50 mM sodium acetate buffer	4.5	40°C	20 µM 4-Methylumbelliferyl N,N',N''-triacetyl-β-chitotrioside	4-Methylumbelliferone	—	—	Classical aqueous control (in %) \| Preincubation of 2 minutes at 40°C in the presence of the organic solvent, but so short that we classify as activity, and assay in the presence of organic solvents

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*