Laccase 1 (gene lcc1) from Trametes trogii

Enzyme Description

Species

Trametes trogii ↗

Extremophile

No but "thermostable" enzyme

EC Number

1.10.3.2 ↗

Sequence

Length: 517 amino acids

MARFQSLLTFITLSLVASVYAAIGPVADLTISNGAVSPDGFSRQAILVNDVFPSPLITGNKGDRFQLNVIDNMTNHTMLKSTSIHWHGFFQHGTNWADGPAFVNQCPISTGHAFLYDFQVPDQAGTFWYHSHLSTQYCDGLRGPIVVYDPQDPHKSLYDVDDDSTVITLADWYHLAAKVGSPVPTADATLINGLGRSIDTLNADLAVITVTKGKRYRFRLVSLSCDPNHVFSIDGHSLTVIEADSVNLKPQTVDSIQIFAAQRYSFVLNADQDVGNYWIRALPNSGTRNFDGGVNSAILRYDGAAPVEPTTSQTPSTNPLVESALTTLEGTAAPGSPAPGGVDLALNMAFGFAGGKFTINGASFTPPTVPVLLQILSGAQSAQDLLPSGSVYSLPANADIEISLPATAAAPGFPHPFHLHGHTFAVVRSAGSSTYNYENPVYRDVVSTGSPGDNVTIRFRTDNPGPWFLHCHIDFHLEAGFAVVMAEDIPEVAATNPVPQAWSDLCPTYDALSPDDQ

Xiaofei Song et al. (2024) — Decolorization and detoxication of malachite green by engineered Saccharomyces cerevisiae expressing novel thermostable laccase from Trametes trogii

Bioresource Technology · doi:10.1016/j.biortech.2024.130591 ↗ · Stability - Incubation

5 measurements ▼

Database ID

UniProt: Q9HDQ0 ↗

Sequence Annotation

Inferred - from protein name

Protein Source

Recombinant, host yeast Saccharomyces cerevisiae 1447

Experimental Data (5 measurements)

5 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	30%	100	88	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	77	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	30%	100	88	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	30%	100	78	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	30%	100	70	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control

Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	30%	100	88	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	30%	100	77	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	30%	100	88	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	30%	100	78	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at ?°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Isopropanol	30%	100	70	%	Incubation: ?, Assay: ? mM citrate buffer	Incubation: ?, Assay: 2.4	Incubation: ?, Assay: 50°C	ABTS diammonium salt, H₂O₂ (Hydrogen Peroxide)	ABTS radical cation diammonium salt	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about protein sequence, uncertainty about whether assay really was in aqueous phase, uncertainty about control

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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