(R)–enantio–selective type IV amine transaminase (gene M16AT) from Mycobacterium sp. ACS1612

Enzyme Description

Species
Mycobacterium sp. ACS1612 β†—
Extremophile
No
EC Number
2.6.1.- β†—

Sequence

Length: 339 amino acids
MTVVDQGTSNLVAVEPGAIREDDTPPGSVIQYSDYELDTSSPYAGGVAWIEGEYIPASEARISIFDTGFGHSDLTYTVAHVWHGNIFRLGDHLDRLLDGAHKLRLDPGYSKDDLAEITKRCVAMSQLRESFVNLTVTRGYGKRKGEKDLSKLTHQVYIYAIPYLWAFPPHEQIFGTTAIVPRHVRRAGRNTVDPTIKNYQWGDLTAASFEAKDRGARTAILMDADNCVAEGPGFNVVIVKDGKLASPSRNALPGITRKTVFELADAMGIEATLRDVTSHELYDADELMAVTTAGGVTPINSLDGEPIGDGTPGPLTVAIRDRFWALMDEPSPLIEAIDY
Tingting Li et al. (2024) β€” Identification, characterization and application of M16AT, a new organic solvent–tolerant ( R )–enantio–selective type IV amine transaminase from Mycobacterium sp. ACS1612
ChemBioChem  Β· doi:10.1002/cbic.202300812 β†—  Β· Activity - Classical
20 measurements ▼
Database ID
NCBI: WP_067809071.1 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100 92 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethylformamide (DMF) 50% 100 0 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethylformamide (DMF) 40% 100 0 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethylformamide (DMF) 30% 100 0 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethylformamide (DMF) 20% 100 0 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethylformamide (DMF) 10% 100 91 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 50% 100 2 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 40% 100 12 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100 56 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100 83 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Acetonitrile 10% 100 101 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Methanol 50% 100 5 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Methanol 40% 100 19 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Methanol 30% 100 70 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Methanol 20% 100 103 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Methanol 10% 100 107 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Acetonitrile 50% 100 2 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Acetonitrile 40% 100 3 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Acetonitrile 30% 100 7 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (acetophenone absorbance measurement, 245 nm) in the presence of organic solvent Acetonitrile 20% 100 70 % 50 mM potassium phosphate buffer 7 40Β°C 20 mM (R)-1-Phenylethylamine, 20 mM Pyruvate Acetophenone , L-Alanine 1 mM PLP β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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