Lipase (gene CPE) from Pseudomonas sp. TB11

Enzyme Description

Species

Extremophile

EC Number

3.1.1.- ↗

Sequence

Length: 615 amino acids

MGVYDYKNLTTEDSKALFADAMAITLYSYHNLDNGFAAGYQQYGFGLGLPATLVQALIGSGTSQGVIPGIPWNPDSEKAALDAVQKAGWTPISASTLDYGGKVDARGTFFGEKAGYTSAQVEVLGKYDDAGKLLEIGIAFRGTSGPRENLILDSIGDVINDLLAAFGPADYAKNYAGQAFGDLLGKVAAYASAQGLSGQDVLVSGHSLGGLAVNSMADLSNNSWAGFYQDANYVAYASPTQSSGDKVLNIGYENDPVYRALDGSSFNLSSLGVHDQPHASSADNIVSFNDHYASTLWNILPFSILNLPTWISHLPTGYGDGMGRILESKFYDLTTRDSTIIVANLSDPARANTWVQDLNRNAEPHKGSTFIIGSDNDDLIQGGRGNDYLEGRAGNDTFRDGGGYNILLGGKGFNTLDLQQSLKNVEVANDGAGTLYIRDGQGGISMTRDMGAVVSKESYLWILSKDVTHSVTDQGLLAGRDLTAYAASLKGDAGANLLKAGDGGKWLFGLDGNDHLIGGKGNDVLVGGAGNDLLEAGGGRNTFLFDGDFGQDRIVGYQSSDKLVFMGVEGVNGQYNYLDHAKAVGSDTLLTFGDNSVTLVGVGLGSLSADGIVIT

Linlin Sha et al. (2024) — Structure-directed bioengineering the lid1 of cold-adapted Pseudomonas sp. TB11 esterase to boost catalytic capacity

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.128302 ↗ · Activity - Classical

14 measurements ▼

Database ID

UniProt: A0A0S2CEE2 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements

Mutation	Property	Assay	Solvent	Solvent Volume	Aqueous Reference	WT Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	—	132	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	—	126	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
T52Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	134	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
T52Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	130	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	144	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	136	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55F	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	136	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55F	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	131	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55V	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	156	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55V	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	142	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	135	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	130	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55R	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	131	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55R	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	124	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in %)

WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	—	132	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
WT	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	—	126	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
T52Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	134	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
T52Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	130	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	144	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55Y	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	136	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55F	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	136	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55F	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	131	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55V	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	156	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55V	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	142	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	135	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55N	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	130	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55R	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	n-Hexane	0.5%	100	132	131	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)
Q55R	Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Cyclohexane	0.5%	100	126	124	%	50 mM Tris-HCl buffer, 0.1 % arabic gum, 0.6 % Triton X-100	8.5	25°C	10 mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in %)

Mutations in this dataset (7)

WT T52Y Q55F Q55N Q55R Q55V Q55Y

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume. — — — Reference value. Hover for details.

Mutation Effect

Mutation impact on enzyme stability and function in the presence of organic solvent: comparison of wild-type and mutant values in identical conditions.

Structure

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