Ξ²-D-glucosidase (gene Bgl1356) from a soil metagenomic library
Enzyme Description
Sequence
MTDRQKNITLNFPKKFLWGAATAAHQVEGGNHNQWSVWELENARTLAVQAEHNVNYVPAWDEIKAEATKPDNYISGQASDHFRQYEKDFDILKKMNMNAFRFSVEWSRIEPNEGAWSAEGITHYKRYIQALKDRGIEPVMTLFHFTLPVWFAEKGGFEKQGNIKYFLRFAEKVLREIGTHVRYVVTINEPEVYATMSYGEGAWPPNKTSKMAKIKVLRNMVTAHNKTAKLAHKVSRRYKVGVAYNVAFHYAGDDALLSQATTKVKQWARDDFFLNAVRRSTDWIGVNYYFSERYYGYRSHNPNNQVSDLGWDMQPENIQFVLERLFAKYGKPLMVTENGVADRNDEYRQWWISKTLQAMHKALKNGVPLEGYLHWSLLDNFEWSYGKWPRFGLIEVDYETGKRRPRKSAAWLARVLKHIQELAAALEHSRVALGTHWLPG
Qian Yao et al. (2024)
β Screening, cloning, immobilization and application prospects of a novel Ξ²-glucosidase from the soil metagenome
Environmental Research
Β· doi:10.1016/j.envres.2023.117676 β
Β· Activity - Classical
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Database ID
UniParc: UPI0024CDC15F β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli
Experimental Data (4 measurements)
4 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Dimethyl Sulfoxide (DMSO) | 15% | 100 | 95 | % | ? | ? | ? | ? mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Methanol | 15% | 100 | 81 | % | ? | ? | ? | ? mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Ethanol | 15% | 100 | 69 | % | ? | ? | ? | ? mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
| Activity - Classical | Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent | Isopropanol | 15% | 100 | 64 | % | ? | ? | ? | ? mM p-Nitrophenyl Ξ²-D-glucopyranoside | D-Glucose , p-Nitrophenol | β | β | Classical aqueous control (in %) |
Visualization : Activity β Classical
One bar per measurement. Colour = solvent, shade = solvent volume.