Oxygenase (gene MoxA) from a deep‐sea sediment metagenomic library

Enzyme Description

Species
Na (deep‐sea sediment metagenomic library)
Extremophile
No
EC Number
1.14.13.- β†—

Sequence

Length: 424 amino acids
MRKITIVGAGQAGLMIGVGLVRKGYDVTIISNQTPEEIRNGRVTSSQGMQATPIAYEREMGLRLWEDDYQPWGGIEFNVLNPETHQKQLTFSTRIGPLNGRPDWVCESIDQRVKMPAWIYKFEELGGKMVYEDADLSALERYETESDLVLVAAGKGEIAKLLVRDEEKSVFDKPMRALGLAYVKGMGPIETRDNGQIRRGLTWNAAPGVGEFFACNALTTSGECDIWIFEGVPGGPMDILDTRDGPEKYFQNCLKVLREYFPDQYARCENTRLTDDLGILSGRFPPTVRKPVMNLPKGGLAMGVADAVVLNDPITGQGSCNAAKFAKVVYDAVLDHGYREFDAQWMQATFDSFWVAAKPVVDWTNSMLVEPGAAQMKLMAAADQNPAIGEFLIRGMDDAGRLMPYWVDEAAADDLIAQLSQQVA
Jikun Du et al. (2023) β€” Characterization of a novel monooxygenase originating from a deep-sea sediment metagenomic library
Applied Microbiology and Biotechnology  Β· doi:10.1007/s00253-023-12719-6 β†—  Β· Activity - Classical
6 measurements ▼
Database ID
UniParc: UPI00016529B4 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 1% 100 61 % 100 mM Tris-HCl buffer 8 30Β°C 1 mM Indole Inoxyl 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100 51 % 100 mM Tris-HCl buffer 8 30Β°C 1 mM Indole Inoxyl 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 1% 100 54 % 100 mM Tris-HCl buffer 8 30Β°C 1 mM Indole Inoxyl 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Methanol 5% 100 40 % 100 mM Tris-HCl buffer 8 30Β°C 1 mM Indole Inoxyl 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 1% 100 41 % 100 mM Tris-HCl buffer 8 30Β°C 1 mM Indole Inoxyl 0.2 mM NADPH β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in the presence of organic solvent Ethanol 5% 100 30 % 100 mM Tris-HCl buffer 8 30Β°C 1 mM Indole Inoxyl 0.2 mM NADPH β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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