Chloroperoxidase from Hortaea werneckii UBOCC‐A‐208029

Enzyme Description

Species

Hortaea werneckii UBOCC‐A‐208029 ↗

Extremophile

Yes halophilic organism

EC Number

1.11.1.10 ↗

Sequence

Length: 627 amino acids

MIPLHQRPEDTDYNKNYVLYWNHVALELVRLTHTETASGAVNGPPLVARMLGILHLAIHDAFFALHNTAGISTYLSPTQSAPYRLPDILDARDGRQAVAGAAITVLEDQYFVSHPSESFSANDQAEQLLRRYITAFAPDTLSSSYRFGYEVGKAMLKLLAIKQDEPGTDQDSYMPSQGQYRFFHDPTNPVVVSSVDPNDPESPKRASRVAHAPFYGMTAKRLAVQYRIGKDQTEHIIADPPVGFGEADMPEYVDALRDVYRMGGRTELNTTTRRPWQTAAAHFWAYDGSNLIGVPLRLYNQILRTVAWDHRPDKRIPDSDKNNVEFARLFALCNAAMADAGIFAWQEKYCFEFWRPLSGVREASTDLIVRNRGNGMVEGIPDPDRPPTMDDGTVALQDPFWLELGAPNTNSNRIPFKPAFPAYPSGHATFGAACFQMMRLYYKNSHRDGIADFDIDGPDNIAFDFVSDEQDGRNRDNLRNPFDPNVPIDDQPGIVRALWKRRFGSLWEALWENAISRVWLGVHWRFDAFSPKDALVRNPTPTTVSSKTDSPLLYAVETDHSTRYKPLAEVRYTSTGTRRDRPGKNFPVGGVPLGIGIANDIFQGGLRPTAQNKQPSGRHRCGRLGKP

Bastien Cochereau et al. (2023) — Heterologous Expression and Biochemical Characterization of a New Chloroperoxidase Isolated from the Deep-Sea Hydrothermal Vent Black Yeast Hortaea werneckii UBOCC-A-208029

Marine Biotechnology · doi:10.1007/s10126-023-10222-7 ↗ · Activity - Classical

5 measurements ▼

Database ID

UniParc: UPI0027A96A98 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (5 measurements)

5 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Acetonitrile	40%	100	35	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O , HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Methanol	40%	100	39	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O , HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	54	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O , HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	74	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O , HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Ethanol	40%	100	84	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O , HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Acetonitrile	40%	100	35	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O, HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Methanol	40%	100	39	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O, HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Dimethylformamide (DMF)	40%	100	54	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O, HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	74	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O, HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (monochlorodimedone and HOBr reaction product absorbance measurement, 290 nm) in the presence of organic solvent	Ethanol	40%	100	84	%	50 mM buffer ?	7	20°C	1.2 mM H₂O₂ (Hydrogen Peroxide), 100 µM KBr	H2O, HOBr	10 µM Na₃VO₄	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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