Laccase-like enzyme from Thermomicrobium roseum DSM 5159

Enzyme Description

Species
Thermomicrobium roseum DSM 5159 β†—
Extremophile
Yes thermophilic organism
EC Number
1.10.3.2 β†—

Sequence

Length: 256 amino acids
MIVRPFRFARLSALGIPHGVTKRDPSLPAFGSVSGNVTISIVLANRRAWWERLGLPLEHTVFARQVHGTRVTVVTASERGRGALEPGTGIPESDALVTTERNLPLAMICADCVPVLLYAPDVPAIGVVHAGWRGTVAGITRQAVHILCTLFSARPERLWAFLGPSIGPCCYEVGEEVITAWLATEPSNGHQAVLSRNGRAIFDLWRANELLLVEGGVDPAHIERAGICTRCHAGEWFSYRANGSRAGAQAAVIALP
Mengyao Hao et al. (2023) β€” Preparation and characterization of a laccase-like enzyme from Thermomicrobium roseum
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2023.124992 β†—  Β· Stability - Incubation
20 measurements ▼
Database ID
UniParc: UPI000191D79F β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Bacillus subtilis WB600

Experimental Data (20 measurements)

20 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Glycerol 10% 100.0 86.3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) Chloroform 20% 100.0 37.0 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Chloroform 10% 100.0 77.9 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Toluene 20% 100.0 50.0 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Toluene 10% 100.0 80.1 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Cyclohexane 20% 100.0 48.0 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Cyclohexane 10% 100.0 71.7 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Ethyl Acetate 20% 100.0 88.2 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Ethyl Acetate 10% 100.0 70.8 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Glycerol 20% 100.0 65.5 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Methanol 10% 100.0 91.8 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase 1-Propanol 20% 100.0 22.2 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase 1-Propanol 10% 100.0 20.8 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100.0 98.5 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100.0 103.7 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Acetonitrile 20% 100.0 66.7 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Acetonitrile 10% 100.0 83.4 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Ethanol 20% 100.0 96.6 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Ethanol 10% 100.0 115.7 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by HPLC (hypoxanthine concentration measurement) in aqueous phase Methanol 20% 100.0 92.3 % Incubation: 50 mM phosphate buffer, Assay: 50 mM phosphate buffer Incubation: 7, Assay: 7 Incubation: 4Β°C, Assay: 37Β°C 15 mM Inosine Hypoxanthine β€” β€” Water-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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