Protease from Mesonia algae K4-1

Enzyme Description

Species
Mesonia algae K4-1 β†—
Extremophile
Yes "psychrophilic" and "moderately halophilic" organism
EC Number
3.4.- β†—

Sequence

Length: 984 amino acids
MKKDYKSLAVMATFLLGSTFGFSQESPKKPIKKLNVYEESQSFQTADDSFKRSLGLPPENTLSLVEQRTDKLGYHHQKFQQYYNSIPVEFATTVLHAKEGKVKSSSNNLYRIKDLPTTPSISKEMAFNKAKQHVGATEYMWEDKEESKHISYNLPAGELVILPKIKGVTEENKLAYKYDIYATAPLYRADVYVDAQTGDFIMENKKIHHANTPSSGTTLYNGNVSITTDSYNGSYRLRQTADGGGIQTYDMNNSTNYNNAQEVTSSSSNFTNNPTAVQAHWGAEQTHKYFSQKHNRNSYNGNGAIIRSYVSYDSNYVNAFWDGSRMTYGDGDGVNYGPLVALDIVGHEIGHGVTEYAANLVYSYESGALNESFSDIFGEAIEAFATGTNDWLMGDEIGSGGSGGALRSISNPNAYGQPDTYLGTSWYSGSGDNGGVHYNSGVQNFWFYLLSTGGSGTNDNGDSYSVTSIGMEKAAAVAYRNLTTYLSSNSQYSDARNGAIQAAKDLYGAGSTEEIAVTNAWHAVGVGAAYSGGGNPEPEDCTSGDIYLSITFDNYPEETSWTLKDASGNTVDSAAYSTSNADGSTVTKTISGLTDGDYTFTITDQYGDGICCSYGSGSYTLSSDDGTISSGGNFGNSEATNFCIEDGGTSLDSEAPSAPSALSANNITQTTVDLSWNASTDNVGVDSYEVFQGSSSLGTTTGTSAQITGLTAASSYSFSVKAKDVAGNTSSSSNVVNVTTTGGNTPDYCNSQGNDSSYEWIDLVSLNNLNNSSGNDGGYADNTNLIANLPYGSNTIQISAGFASSSYTEHWAVYIDYNQNGAFETNEKVVSGSSSSGNTLSGTFSVPTSAISGNTRMRVSMKYNASQTSCESFSYGEVEDYTVNVGQAAAQGFNNNSLAATAIGNESAIFATTIYPNPVTGNEIFVKMEDARDATYKIYDMLGKLVLSGELTQNAIQLNRLSGGVYMLQLGDGQKSITKKFVKQ
Hailian Rao et al. (2023) β€” Characteristics and Application of a Novel Cold-Adapted and Salt-Tolerant Protease EK4-1 Produced by an Arctic Bacterium Mesonia algae K4-1
International Journal of Molecular Sciences  Β· doi:10.3390/ijms24097985 β†—  Β· Stability - Incubation
9 measurements ▼
Database ID
UniParc: UPI0011C72452 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Purified, bacterium Mesonia algae K4-1

Experimental Data (9 measurements)

9 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase Ethanol 25% 100 24 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase Isopropanol 25% 100 97 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase Methanol 25% 100 8 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase Isoamyl Alcohol 25% 100 18 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100 48 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase Xylene 25% 100 70 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase 1-Propanol 25% 100 81 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase n-Hexane 25% 100 70 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (12 hours at 4Β°C), measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagent and free tyrosines reaction product absorbance measurement, 660 nm) in aqueous phase 1-Butanol 25% 100 33 % Incubation: ?, Assay: 20 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 4Β°C, Assay: 40Β°C 2% (w/v) Casein free amino acids , Peptides β€” β€” Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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