Glucose dehydrogenase (gene BcGDH90) from Bacillus cereus HBL-AI

Enzyme Description

Species
Bacillus cereus HBL-AI β†—
Extremophile
No
EC Number
1.1.1.47 β†—

Sequence

Length: 261 amino acids
MYSDLAGKVVVITGSATGLGRAMGVRFAKEKAKVVINYRSRESEANDVLEEIKKVGGEAIAVKGDVTVESDVVNLIQSAVKEFGTLDVMINNAGIENAVPSHEMPLEDWNRVINTNLTGAFLGSREAIKYFVEHDIKGSVINMSSVHEKIPWPLFVHYAASKGGIKLMTETLALEYAPKGIRVNNIGPGAINTPINAEKFADPKKRADVESMIPMGYIGNPEEIAAVATWLASSEASYVTGITLFADGGMTLYPSFQAGRG
Xiaozheng Li et al. (2023) β€” Characterization and Application of a Novel Glucose Dehydrogenase with Excellent Organic Solvent Tolerance for Cofactor Regeneration in Carbonyl Reduction
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-023-04432-x β†—  Β· Stability - Incubation
8 measurements ▼
Database ID
UniParc: UPI000018ED4B β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 90% 100.0 0.0 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Acetonitrile 90% 100.0 36.5 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Acetone 90% 100.0 84.7 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Methanol 90% 100.0 88.7 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Ethanol 90% 100.0 102.9 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase 1-Propanol 90% 100.0 75.5 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase Isopropanol 90% 100.0 121.7 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (1 hour at 25Β°C), measured by absorbance spectrophotometry (NADH absorbance measurement, 340 nm) in aqueous phase 1-Butanol 90% 100.0 0.0 % ? Incubation, 30 mM HEPES buffer Incubation: ?, Assay: 7 Incubation: 25Β°C, Assay: 30Β°C 100 mM D-Glucose D-glucono-1,5-lactone 2 mM NAD+ β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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