Xylanase (gene PersiXyn10) from a camel rumen microbiota metagenomic library

Enzyme Description

Species

Na (camel rumen microbiota metagenomic library)

Extremophile

Yes "hyperthermostable" enzyme

EC Number

3.2.1.8 ↗

Sequence

Length: 354 amino acids

MNSKMVGATALCVAFIASGAYAQDFCNAKHSGSKKTVSFSVGAGADKGETGPIGEYHYEQWNKSGSATTDFYEDGSFSCSFSNTDDFLCREGVFYGQNSGKDPLSVGNLVADYSIQTFTNDKSISYAYAGIYGWMQNPLIEWYIVDNWGPASRPNWLGTSRGKITVDGAEYEIFTASANRATIEGYKQFDQIYSLRATARKCGTISITEHFKAWKSKGIALGSSLYEAKILGEAGQYPEQQGASGKIDFNYAKVYVQSEPKSSSSTTPTSSAIAPTSSSSVVNSSSTIALPVERADFRMAGSAQVFDMLGKYLGTVELNAGVSVKELLLTKFGKSGTFLLKRGASSTVISVENR

Shohreh Ariaeenejad et al. (2023) — Efficient bioconversion of lignocellulosic waste by a novel computationally screened hyperthermostable enzyme from a specialized microbiota

Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2023.114587 ↗ · Stability - Incubation

8 measurements ▼

Database ID

UniParc: UPI0027A7A293 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (8 measurements)

8 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethylene Glycol	50%	100.0	102.08	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	50%	100.0	88.39	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	50%	100.0	113.2	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	50%	100.0	108.37	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetonitrile	50%	100.0	93.56	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	79.42	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isobutanol	50%	100.0	132.56	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	100.0	116.44	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control

Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethylene Glycol	50%	100.0	102.08	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Ethanol	50%	100.0	88.39	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Methanol	50%	100.0	113.2	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isopropanol	50%	100.0	108.37	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetonitrile	50%	100.0	93.56	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	50%	100.0	79.42	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Isobutanol	50%	100.0	132.56	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase	Acetone	50%	100.0	116.44	%	Incubation: ?, Assay: ? Mm phosphate buffer	Incubation: ?, Assay: 8	Incubation: Room temperature, Assay: 90°C	1% Soluble birchwood xylan	Reducing sugars	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*