Lipase (gene PersiLipase1) from a tannery wastewater metagenomic library

Enzyme Description

Species
Na (tannery wastewater metagenomic library)
Extremophile
No but "thermostable" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 322 amino acids
MDTNNYPTVENKTKLFLEKLQQSGGPPLYTLTPTEARNVLSGLQAGPIEKLPAEIENKTIPGGPNGEISIQIVRPQGSGNETLPVVMYTHGGGWVLGNFNTHERLLRELSTGAHAAIVFVNYTLSPEAKYPQSLEEAYAATKWISENGQSLNLDSSRLVVAGDSVGGNMATTLALLAKERGGPPITFQLLFYPVTDANFNTSSYNTYQEGYFLTRENMKWFWDNYVSKDTNRKEPTVSPLQASLEQLSGLPPTLIIVGENDVLRDEGEAYAHKLMQAGVPITATRYLGAIHDFMMLNPISDTPAARGAIDQASHTLKELFSK
Shohreh Ariaeenejad et al. (2023) β€” Efficiency of an alkaline, thermostable, detergent compatible, and organic solvent tolerant lipase with hydrolytic potential in biotreatment of wastewater
Science of The Total Environment  Β· doi:10.1016/j.scitotenv.2022.161066 β†—  Β· Stability - Incubation
12 measurements ▼
Database ID
UniParc: UPI002187654C β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10% 100 266 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 20% 100 209 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 10% 100 176 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 20% 100 210 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 10% 100 236 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 20% 100 276 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 10% 100 189 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Acetone 20% 100 180 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100 188 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 20% 100 252 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 10% 100 74 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 25Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Isopropanol 20% 100 72 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 9 Incubation: 30Β°C, Assay: 60Β°C ? mM p-Nitrophenyl laurate Lauric Acid , p-Nitrophenol β€” β€” Non-incubated control (in %), assay in aqueous phase | High uncertainty about whether assay really was in the presence of organic solvent or in aqueous phase, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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