GH3 β-xylosidase (gene AmβXyl) from Alicyclobacillus mali FL18

Enzyme Description

Species

Alicyclobacillus mali FL18 ↗

Extremophile

Yes "thermo-acidophilic" organism

EC Number

3.2.1.37 ↗

Sequence

Length: 779 amino acids

MYLDPAQSVDARVDALLADMTLEEKVAQLTSIWAFEILDDLRFSAEKAAAVLGQGIGQVTRIGGATNLDPPDVARLANQIQRYLRDHTRLGIPALIHEESCSGYMAKGATCFPQTIGIASTWDVDLAQRIGAIIRDQMRAVGARQALAPLLDVARDPRWGRVEETFGEDPYLVAQMGIAYVRGLQGEDLSQGVMATGKHFVGYGASEGGMNWAPAHIPERELREVYLFPFEAAVREAGLGAIMPGYHELDGVPCHDNPGLLRETLRGRWGFQGLVVSDYFAVNQLFEYHQVARDKAEAAALAVRAGVDVELPTRDVYGKPLIEAVARGLVSVAEIDELVRRVLTWKFRLGLFDHPFVDEGAAIAVFDNAEQRKVAREAAEKSLVLLKNDGLLPLAPRGTIAVIGPNAHTTRNLVGDYAYPCHIESLLEQSEDNVFQTPLPSGVKHVDEFIPMRTILEAIGHRVGSEAQVVYAKGCDVLEGEDADLDEAVALAAQSDVAVVVVGDRAGLTDACTTGESRDRATLSLIGRQEELVRRVLATGTKTVVVLVSGRPLAIPDIAERANAVLEAWLPGEEGAEAVAAVLFGDVNPSGKLPVTIPRSVGQVPIYYGHKPSGGRSHWKGAYVDESNLPLYPFGHGLSYTAFEYRDLTLSASELGVHGEIEVACVIENVGARAGEEVVQLYARDVAADVTRPVKVLCGFARVPLAPGEKARVRFRVSAHQFGFYNREMRYVVEPGEIEIMVGASSEDIRLRGMVRIHGAMTEIEHEKVYQSAVNVERM

Flora Salzano et al. (2022) — Alicyclobacillus mali FL18 as a Novel Source of Glycosyl Hydrolases: Characterization of a New Thermophilic β-Xylosidase Tolerant to Monosaccharides

International Journal of Molecular Sciences · doi:10.3390/ijms232214310 ↗ · Activity - Classical

18 measurements ▼

Database ID

UniProt: A0ABS0F573 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	40%	100	562	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	60%	100	221	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	50%	100	490	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	40%	100	778	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	30%	100	1246	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	1065	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	482	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	60%	100	126	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100	357	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	222	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100	600	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	847	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100	612	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100	15	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	110	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	280	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	461	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	387	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	40%	100	562	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	60%	100	221	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	50%	100	490	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	40%	100	778	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	30%	100	1246	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	100	1065	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	100	482	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	60%	100	126	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	50%	100	357	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	222	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	30%	100	600	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	20%	100	847	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Ethanol	10%	100	612	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	60%	100	15	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	50%	100	110	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	40%	100	280	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	461	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	387	%	50 mM sodium citrate, 1 mM DTT	5.6	60°C	4 mM p-nitrophenyl β-D-xylopyranoside	D-Xylose, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

🧬

No structure available for this protein.