β-glucosidase (gene Tsbgl) from Thermoclostridium stercorarium DSM 8532

Enzyme Description

Species

Thermoclostridium stercorarium DSM 8532 ↗

Extremophile

Yes "thermophilic" organism

EC Number

3.2.1.21 ↗

Sequence

Length: 751 amino acids

ISQMTLEEKASLCSGLDAWNLKSVERLGIPSIMVSDGPHGLRKETTDPTDPGKKTTVPATCFPTAVGLASSWNRELVEKVGAALGEECQAEGIAVLLGPGTNIKRSPLCGRNFEYFSEDPYLSSEMAASHIKGVQSRGVGTSLKHFAANNQEHRRMSVDAVIDERTLREIYLASFEGAVKKAKPWTIMCSYNRVNGEYASENKFLLTDVLRNEWGFEGIVVSDWGAVNERVKGLEAGLDLEMPSSFGIGDQKIVEAVKKGELPEEVLDRTVERILNLIFKAVDNRKENAGYDREAHHKLAREAARECMVLLKNEDKILPLRKQGTIAVIGEFAKRPRYQGGGSSHVNPTIMDSPYEEIKKSAGNNADVIYAQGYFIEKDEPDEKLLEEAKQAALKADVAVIFAGLPEHYECEGYDRQHMRMPESHCTLIEEVAKVQPNVVVVLCNGSPVEMPWIDKVKGLLEAYLGGQAMGGAIADLLFGDANPSGKLAETFPKQLSDNPSYLNFPGEGDRVEYREGIFVGYRYYDKKNMEPLFPFGYGLSYTTFEYGDLKISRKEISDNETVTVSVKVKNTGDMAGKEIVQLYVRDIESSVIRPEKELKGFEKVELKPGEEKTVVFELDKRAFAYYNTGIRDWHVETGEFEILIGRSSRDIVLKDKIFVKSTVTIKKPVDRNTLVGDLLSDRVLEPVFREFIINEIKNRYLLDLLENEDKSLLSVWMRYTPLRSLANSTGGELNEEKLNRLIDTLNANIK

Cheng Zeng et al. (2023) — Specific and efficient hydrolysis of all outer glucosyls in protopanaxadiol type and protopanaxatriol type ginsenosides by a β-glucosidase from Thermoclostridium stercorarium

Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2022.110152 ↗ · Activity - Classical

18 measurements ▼

Database ID

UniParc: UPI0002AD7EC8 ↗

Sequence Annotation

Explicit - Provided (7 first residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (18 measurements)

18 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	78	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	100	0	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	25%	100	0	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100	15	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	15%	100	35	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	46	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	5%	100	68	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	70	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	72	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	5%	100	98	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	88	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	93	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	95	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	100	6	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	25%	100	11	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	100	26	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	15%	100	47	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	85	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose , p-Nitrophenol	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	100	78	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	30%	100	0	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	25%	100	0	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	20%	100	15	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	15%	100	35	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	10%	100	46	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Ethanol	5%	100	68	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	30%	100	70	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	72	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	5%	100	98	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	15%	100	88	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	100	93	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	5%	100	95	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	30%	100	6	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	25%	100	11	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	20%	100	26	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	15%	100	47	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in the presence of organic solvent	Methanol	10%	100	85	%	50 mM sodium acetate buffer	5	65°C	1 mM p-Nitrophenyl β-D-glucopyranoside	D-Glucose, p-Nitrophenol	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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