GH42 β-galactosidase (gene btgal42) from Bifidobacterium thermophilum

Enzyme Description

Species

Extremophile

EC Number

3.2.1.23 ↗

Sequence

Length: 691 amino acids

MTARRTHRWPRPLDNAARRIWYGGDYNPEQWPESVWDEDIELMNQAGVNIVSLGIFAWSAIEPEEGVYDFGWLDRIIDKLYHAGIAVDLASATASPPLWLTQKHPEVLWKDERGDACWPGARQHWRPTSPIFREYALRLCRAMAQHYRDNPAVVAWHVSNEYGCHNRFDYSDDALRAFQRWCKERYGDIEAVNDAWGTYFWSQRMTDFSQIIPPRYIGEGNFMNPGKLLDFKRFCSDALKEFFKAERDALAEITPNIPLTNFMVSAPGNALDYDDWGDEVDFVSNDHYFTPGRRHFDELAYSSSLVDGISRKQPWFLMEHSTSAVNWRSINYRKEPGQLERDAMAHLAMGADAICFFQWRQSQAGAEKFHSGMVPHAGRNSQVYRDVCALGNDLDTSQAGLPGTTLSQARIAVVYDYASEWATEHTATPTQQVRHWTEPLDWFTALADQGLTADVVPLAGDWDRYEMVVLPSVYLLGENDARRVRDYVAAGGKLATYYTGISDERDHVWLGGYPGAIRDVVGVRSEEFAPMGDDDGVDHLDLSNGTVAHDIADVITSTADSARILATYQADSWTGMNGVPAITVNTYGEGRAAYVGCRLGADGLAASLPAMFEAMDVSVPAWQGSGDILRVVREGRGDGEHEAPRFTFQFNRTHHPVAADIEGRTIVASLAEDHGDGTATIAPNGVIVSMG

Jun Duan et al. (2022) — A GH42 β-galactosidase from Bifidobacterium thermophilum : Biochemical characterization and synthesis of galactosyl glycerol by reverse hydrolysis

Biotechnology and Applied Biochemistry · doi:10.1002/bab.2408 ↗ · Stability - Incubation

6 measurements ▼

Database ID

NCBI: AGH41516.1 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Glycerol	10%	100.0	89.1	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100.0	81.3	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	10%	100.0	82.7	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethylene Glycol	10%	100.0	84.3	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	10%	100.0	82.6	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	10%	100.0	80.7	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose , o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control

Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Glycerol	10%	100.0	89.1	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Methanol	10%	100.0	81.3	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	10%	100.0	82.7	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethylene Glycol	10%	100.0	84.3	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	10%	100.0	82.6	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation	Activity after incubation (1 hour at 50°C), measured by absorbance spectrophotometry (o-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	10%	100.0	80.7	%	Incubation: ? mM sodium phosphate buffer, Assay: ? mM sodium phosphate buffer	Incubation: ?, Assay: 7	Incubation: 50°C, Assay: 50°C	? mM o-Nitrophenyl-β-D-galactopyranosidee	D-Glucose, o-Nitrophenol	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase, uncertainty about control

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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