Aldehyde dehydrogenase (gene ALDH) from Anoxybacillus geothermalis D9

Enzyme Description

Species
Anoxybacillus geothermalis D9 β†—
Extremophile
Yes "thermophilic" organism
EC Number
1.2.1.3 β†—

Sequence

Length: 505 amino acids
MIYNRPGENGAKVQFKKRYDNYINGEWTPPISGQYFENITPVTGEVFCEVARSQAEDIELALDAAHAAKEAWGKTSPAFRATILNKIADRMEENLEMLAVAETWENGKPIRETLNADLPLAIDHFRYFAGVIRAQEGSISQIDDDTVAYHFHEPLGVVGQIIPWNFPLLMAAWKIAPALAAGNCIVLKPAEQTPASIMVLMELIGDLLPPGVLNIVNGFGLEAGKPLASSPRIAKIAFTGETTTGRLIMQYASQNIIPVTLELGGKSPNIFLPDVMSEEDDFLDKALEGFTMFALNQGEVCTCPSRALIHESIYAAFMERALERVKNIKAGNPLDTSTMIGAQASSEQMEKILSYIDIGKQEGAECLIGGDRNHIAGLENGYYIQPTVFKGHNKMRIFQEEIFGPVVSVTTFKSEEEALAIANDTLYGLGAGVWTRNIHTAYRLGRGIQAGRVWTNCYHAYPAHAAFGGYKMSGIGRETHKMMLAHYQQTKNLLVSYHPKAQGFF
Nur Ezzati Rosli et al. (2022) β€” Structure Prediction and Characterization of Thermostable Aldehyde Dehydrogenase from Newly Isolated Anoxybacillus geothermalis Strain D9
Microorganisms  Β· doi:10.3390/microorganisms10071444 β†—  Β· Stability - Incubation
11 measurements ▼
Database ID
UniProt: A0A7T6X3I8 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli Transetta (DE3)

Experimental Data (11 measurements)

11 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 25% 100.0 55.21 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Methanol 25% 100.0 65.41 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase 1-Propanol 25% 100.0 160.26 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Isopropanol 25% 100.0 102.0 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Benzene 25% 100.0 175.11 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Toluene 25% 100.0 25.11 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase 1-Octanol 25% 100.0 82.43 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase Xylene 25% 100.0 52.66 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase n-Hexane 25% 100.0 55.97 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase n-Heptane 25% 100.0 101.26 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control
Stability - Incubation Activity after incubation (30 minutes at 60Β°C), measured by absorbance spectrophotometry (NADPH absorbance measurement, 340 nm) in aqueous phase n-Tetradecane 25% 100.0 64.47 % Incubation: ?, Assay: 50 mM Tris-HCl buffer Incubation: ?, Assay: 8 Incubation: 60Β°C, Assay: 60Β°C 50 mM Acetaldehyde Acetate 20 mM NADH Incubation: 50 rpm Non-incubated control (in %), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase, uncertainty about control

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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