Ξ²-D-glucosidase (gene SpBGL1) from Sphingomonas sp. ATCC31555

Enzyme Description

Species
Sphingomonas sp. ATCC31555 β†—
Extremophile
No
EC Number
3.2.1.21 β†—

Sequence

Length: 801 amino acids
MRSLRRFAAIAIAASALTPFVASQPASAQTQAARAEKPLYKDASQPTEARVNDLLARMTLEEKVQQLVALWLNKGSIQDTKGEFDAAKASANFPNGLGMISRPYDRRGVTASESTAAGANAGDVNRSPEETARYVYAAQKWAVEKTRLGIPMLMHEEALHGLVAPGATSFPQSIGLASSFNPKLVEQIFSMAAKEARARGANLVLAPVVDVARDPRWGRIEETYGEDPYLVTQMGLAAIRGFQGSTLPLKSDKVFITLKHMTGHGQPENGTNIGPASLGERTLRENFFPPFEAAVKTLPVMSVMASYNEIDGIPSHANKWLLTDVLRGEWGFQGAVVSDYFAIRELMTRHHMYKDPKDAALRAIDAGVDVETPDGEAYANLVALVKEGRLSQGAIDNAVRRVLRMKFEGGLFENPYPEVKLAARTNTPEAVALARQAARESIVLLKNAQGLLPLDAKGIKRMAVIGHTHAKDTPIGGYSDVPNHVVSVLEGMQNEAKGKFAVDFAEGVRITDHRVWSKDEVKLVPDSVNDQLRAQALETAKNADVVVLVLGGNEAISREAWADEHLGDSETLDLPGPQDQLAKELFALGKPVVVILLNGRPYAVNHLAEKAPALIEGWYLGEQTGTALADVVFGRYNPGGKLPVSIARNVGQLPIYYNKKPSARRGYLFGDTTPLYPFGYGLSYTTFDISAPRLGAPVIGIADKAMVEVDVTNTGKVAGDEVVQLYVRDDLASVTRPLLELKRFERVTLKPGERRTVRFELTPDDLALWNSEMKRVVEPGTFTLSAGPNSATLKSATLTVR
Jie Zhou et al. (2022) β€” Application of Ξ²-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
Molecules  Β· doi:10.3390/molecules27051514 β†—  Β· Activity - Classical
12 measurements ▼
Database ID
NCBI: WP_019370396.1 β†—
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli JM109

Experimental Data (12 measurements)

12 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 5% 100 71 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 10% 100 64 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 15% 100 59 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 20% 100 37 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 25% 100 6 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Methanol 30% 100 4 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 5% 100 120 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 10% 100 115 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 15% 100 91 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 20% 100 69 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 25% 100 35 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in aqueous phase Ethanol 30% 100 7 % McIlvaine buffer 6 40Β°C ? mM p-Nitrophenyl Ξ²-D-glucopyranoside D-Glucose , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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