Laccase B (gene Lac1) from Trametes hirsuta MX2

Enzyme Description

Species
Trametes hirsuta MX2 β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 520 amino acids
MAGLQRFGVFVALALVGSAFAAIGPKANLVITDAAIAPDGFLRDAIVTNGVFPAPLIKGNKGDRFQLNVIDNLTNHTMLKSTSIHWHGFFQAGTNWADGPAFVNQCPIASGHSFLYDFHVPDQAGTFWYHSHLSTQYCDGLRGPFVVYDPKDPHASRYDVDNESTVITLSDWYHAAARLGPRFPLGADSTLINGLGRSSSTPNAGLAVINVQRGKRYRFRLVSLSCDPNYTFSIDGHNLTVIEVDGINSKPLTVDSIQIFAAQRYSFVLNANQPVANYWVRANPNFGTTGFAGGINSAILRYQGAPNAEPTTTQTPSVIPLIETNLHPLAKMPVPGKATSGGVDKALNLAFSFNGTNFFINNATFTPPTVPVLLQILSGAQTAQDLLPAGSVYPLPAHSSIEITLPASAAAPGAPHPFHLHGHAFAVVRSAGSTAYNYADPIWRDVVSTGTPAAGDNVTIRFRTDNPGPWFLHCHIDFHLEAGFAVVFSEDVPDVKAANPVPQAWSELCPIYDKLAEGDL
Yitong Jia et al. (2022) β€” Characterization of a Recombinant Laccase B from Trametes hirsuta MX2 and Its Application for Decolorization of Dyes
Molecules  Β· doi:10.3390/molecules27051581 β†—  Β· Activity - Classical
18 measurements ▼
Database ID
UniProt: A0A6M5CX58 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host yeast Pichia pastoris GS115

Experimental Data (18 measurements)

18 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 10% 100.0 53.3 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 15% 100.0 3.0 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 15% 100.0 7.0 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 15% 100.0 4.0 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 15% 100.0 4.3 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 15% 100.0 9.0 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 15% 100.0 0.4 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 10% 100.0 42.5 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 10% 100.0 62.4 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 5% 100.0 69.1 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 43.9 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 10% 100.0 61.3 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetone 10% 100.0 44.2 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Isopropanol 5% 100.0 65.2 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Methanol 5% 100.0 86.2 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Ethanol 5% 100.0 70.0 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 5% 100.0 64.5 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in the presence of organic solvent Acetonitrile 5% 100.0 80.0 % 85 mM citrate buffer 5 30Β°C 0.4 mM ABTS ABTS radical cation β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate Β· Scroll to zoom Β· Right-click drag to pan Β· Powered by Mol*