Lipase sequence construced by ancestral sequence reconstruction of the I.3 family

Enzyme Description

Species

Na (ancestral sequence reconstruction)

Extremophile

No t applicable (ancestral sequence reconstruction)

EC Number

3.1.1.3 ↗

Sequence

Length: 632 amino acids

MGVYDYKNNFGTADSKALFSDAMAITLYSYHNLDNGFAAGYQHNGFGLGLPATLVTALLGGTDSQGVIPGIPWNPDSEKLALEAVKKAAGWTPITASQLGYDGKTDARGTFFGEKAGYTTAQVEILGKYDAQGHLTEIGIAFRGTSGPRENLILDSIGDVINDLLAAFGPKDYAKNYVGEAFGNLLNDVVAFAKANGLSGKDVLVSGHSLGGLAVNSMADLSGGKWGGFFADSNYIAYASPTQSSATSDKVLIGYENDPVFRALDGSTFTGASVGWVHDAPKESATDNIVSFNDHYASTAWNLLPFSILNIPTWISHLPTAYGDGMNRVIESKFYDLTSKDSTIIVANLSDPARANTWVQDLNRNAETHKGSTFIIGSDSNDLIQGGSGNDYLEGRAGNDTFRDGGGYNIILGGQGNNTLDLQKSVNTFDFANDGAGNLYIRDANGGISITRDIGSIVTKEPGFIPWTSILWGLFKDDVTHSVTASGLKVGNNVTQYESSVKGTNGADTLKAHAGGDWLFGLDGNDHLIGGGAGNDVFVGGAGNDLMESGGGGADTFLFNGFHTAFGQDRVVGFTSNDKLVFLGVQGVLPGDDFRAHASAVGQDTVLKFGGDSVTLVGVALNSLSADGIVIA

Mohamad Farihan Afnan Mohd Rozi et al. (2022) — Ancestral sequence reconstruction of ancient lipase from family I.3 bacterial lipolytic enzymes

Molecular Phylogenetics and Evolution · doi:10.1016/j.ympev.2021.107381 ↗ · Stability - Incubation

20 measurements ▼

Database ID

—

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (20 measurements)

20 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	25%	100.0	62.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexadecane	25%	100.0	103.6	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Tetradecane	25%	100.0	107.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Octane	25%	100.0	102.6	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isooctane	25%	100.0	107.2	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Heptane	25%	100.0	101.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Decanol	25%	100.0	43.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Xylene	25%	100.0	84.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Octanol	25%	100.0	40.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Heptanol	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100.0	128.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	25%	100.0	50.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Propanol	25%	100.0	4.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Diethyl Ether	25%	100.0	121.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isoamyl Alcohol	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Pyridine	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	25%	100.0	101.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	25%	100.0	13.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	25%	100.0	125.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol , Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase

Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Toluene	25%	100.0	62.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Hexadecane	25%	100.0	103.6	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Tetradecane	25%	100.0	107.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Octane	25%	100.0	102.6	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isooctane	25%	100.0	107.2	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	n-Heptane	25%	100.0	101.8	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Decanol	25%	100.0	43.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Xylene	25%	100.0	84.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Octanol	25%	100.0	40.7	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Heptanol	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Dimethyl Sulfoxide (DMSO)	25%	100.0	128.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Benzene	25%	100.0	50.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Propanol	25%	100.0	4.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Diethyl Ether	25%	100.0	121.4	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isoamyl Alcohol	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	1-Butanol	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Pyridine	25%	100.0	0.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Ethanol	25%	100.0	101.1	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Isopropanol	25%	100.0	13.3	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (30 minutes at 37°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase	Acetonitrile	25%	100.0	125.0	%	Incubation: ?, Assay: 50 mM Tris-HCl buffer, 2.5% isopropanol	Incubation: ?, Assay: 8	Incubation: 37°C, Assay: 30°C	0.125 mM p-Nitrophenyl palmitate	p-Nitrophenol, Palmitic Acid	—	—	Non-incubated control (in %), assay in aqueous phase \| High uncertainty about whether assay really was in aqueous phase

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*