Ξ±-amylase (gene PersiAmy1) from a cow dung metagenomic libary
Enzyme Description
Sequence
MKKYLLPILCLLAVACGGNGKTALTSIPAPEDVVMYQINPRNFAPDHSFQAVSARLDAIQELGANVVWFMPICEIGVEKAVQSPYCVKNYTAVNPEFGTLEDFKALVDGAHQRGMAVIIDWVANHTSWDNPWIKDHPEWYTHNEAGDIISPAGTGWNDVADLNFDNPDLCQAMIDAMKFWVEEVGVDGFRCDAADYVPFEFWKDCVAQLRATGHELLMLAEGQRKDHFDADFDMNYAWGWLSALRRVYTGVTETIQQPVPQRQQGNRQGQNARQGNRPQGQQPQMRTRTVNRAVPVSTLFASDSSEYAGLPAGRVKLRFTTNHDEHVKNSPVREFFGNDGSVAAFVATTFIHGGMLIYGCQEVGYPGKINFFRYAEIDWDANPDMFNAYKQIVRVFKETPAIRRGALVPYPHNDILIFERVLDNEKVLVMVNMRDTQLEAPVPAAWQGRTATDLITRKSVSFGETERLRPFEYVIVK
Shohreh Ariaeenejad et al. (2021)
β Highly Efficient Computationally Derived Novel Metagenome Ξ±-Amylase With Robust Stability Under Extreme Denaturing Conditions
Frontiers in Microbiology
Β· doi:10.3389/fmicb.2021.713125 β
Β· Stability - Incubation
▼
Database ID
NCBI: MT560082.1 β
Sequence Annotation
Explicit - Provided GenBank Accession Number
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (2 measurements)
2 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Ethanol | 10 mM | 100 | 102 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 10Β°C | 1% (w/v) Starch | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (DNS assay, 540 nm) in aqueous phase | Methanol | 10 mM | 100 | 114 | % | Incubation: ?, Assay: 50 mM Tris-HCl buffer | Incubation: ?, Assay: 9 | Incubation: Room temperature, Assay: 10Β°C | 1% (w/v) Starch | Reducing sugars | β | β | Non-incubated control (in %), assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.