α‐L‐rhamnosidase (gene Rha-N1) from Aspergillus niger

Enzyme Description

Species
Aspergillus niger β†—
Extremophile
No
EC Number
3.2.1.40 β†—

Sequence

Length: 656 amino acids
MWSSWLLSALLATEALAVPYEEYILAPSSRDLAPASVRQVNGSVTNAAALTGAGGQATFNGVSSVTYDFGINVAGIVSVDVASASSDSAFIGVTFTESSMWISSEACDATQDAGLDTPLWFAVGQGAGLYTVEKKYNRGAFRYMTVVSNTTATVSLNSVKINYTASPTQDLRAYTGYFHSNDELLNRIWYAGAYTLQLCSIDPTTGDALVGLGVITSSETISLPQTDKWWTNYTITNGSSTLTDGAKRDRLVWPGDMSIALESVAVSTEDLYSVRTALESLYALQKPDGRLPYAGKPFFDTVSFTYHLHSLVGAASYYQYTGDRAWLTRYWGQYKKGVQWALSSVDSTGLANITASADWLRFGMGAHNIEANAILYYVLNDAISLAQTLNDNAPIRNWTTTAARIKTVANELLWDDKNGLYTDNETTTLHPQDGNSWAVKANLTLSANQSAIVSESLAARWGPYGAPAPEAGATVSPFIGGFELQAHYQAGQPDRALDLLRLQWGFMLDDPRMTNSTFIEGYSTDGSLAYAPYTNTPRVSHAHGWATGPTSALTIYTAGLRVTGPAGATWLYKPQPGNLTQVEAGFSTRLGSFASSFSRSGGRYQELSFSTPNGTTGSVELGDVSGQLVSDRGVKVQLVGGKASGLQGGKWKLSNN
Hangyu Ye et al. (2022) β€” Homologous Expression and Characterization of Ξ±-L-rhamnosidase from Aspergillus niger for the Transformation of Flavonoids
Applied Biochemistry and Biotechnology  Β· doi:10.1007/s12010-022-03894-9 β†—  Β· Activity - Classical
15 measurements ▼
Database ID
UniProt: A0AAJ8E4Z5 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Aspergillus niger AGL-1

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 10% 100.0 64.3 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 20% 100.0 52.4 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Methanol 30% 100.0 44.8 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 10% 100.0 69.2 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 20% 100.0 46.8 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Ethanol 30% 100.0 44.6 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 10% 100.0 87.5 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 20% 100.0 84.2 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Acetonitrile 30% 100.0 66.9 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 10% 100.0 86.7 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 20% 100.0 77.4 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethylformamide (DMF) 30% 100.0 54.4 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 10% 100.0 84.8 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 20% 100.0 71.9 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent Dimethyl Sulfoxide (DMSO) 30% 100.0 68.2 % ? mM phosphate citrate buffer 4.5 60Β°C 0.2 mM p-Nitrophenylrhamnoside L-Rhamnose , p-Nitrophenol β€” β€” Classical aqueous control (in %)

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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