Laccase (gene pLacSi) from Sulfitobacter indolifex

Enzyme Description

Species
Sulfitobacter indolifex β†—
Extremophile
No
EC Number
1.10.3.2 β†—

Sequence

Length: 429 amino acids
MNRRQFLASASALAILPLQASAAADPLKLTAEAVTRQLLPKSEGTTAMLGFNGSMPGPELRLKRGEQISIDVENRLEEGTAVHWHGIRLENRMDGVPVLTQDLINPGDSKTYSFAPPDAGTYWYHSHYISQEQVARGMMGPLIVEDDMPPDVDHDITVVLSDWIINEDGSLSDDFTDMHSVAHAGYMGNFARAFLSQAEVKEGDRVRFRIINAATNRIFPLTVAGVAGSVVALDGMALGQPRGLSDLTLAPAQRADLIVDITGPVSFDMHSRQGSYRLADVAVSGSNTDRKPEPIAPLAAPNLPVPGDPTQHLTLTMMGGAMGGRHGGANIWSFNDVSDLPDAPFASMKRGETVRITLANDTAFPHGIHLHGHHFYELAADGSLGDLRDTTLVAAGESRDVLCVFDNPGRWLIHCHMLSHAIGGMRTWV
Nabangshu Sharma et al. (2021) β€” Characterisation and optimisation of a novel laccase from Sulfitobacter indolifex for the decolourisation of organic dyes
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2021.09.003 β†—  Β· Stability - Incubation
15 measurements ▼
Database ID
β€”
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 10% 100.0 27.2 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 30% 100.0 7.2 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Methanol 50% 100.0 4.6 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 10% 100.0 96.2 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 30% 100.0 7.0 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Ethanol 50% 100.0 3.5 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 10% 100.0 36.3 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 30% 100.0 7.6 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetone 50% 100.0 0.2 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 10% 100.0 32.3 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 30% 100.0 3.3 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Acetonitrile 50% 100.0 5.5 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 100.0 93.3 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 30% 100.0 45.1 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase
Stability - Incubation Activity after incubation (6 hours at room temperature), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 50% 100.0 12.2 % Incubation: 40 mM Britton-Robinson buffer, Assay: 40 mM Britton-Robinson buffer Incubation: 6, Assay: 6 Incubation: Room temperature, Assay: 30Β°C 1 mM ABTS ABTS radical cation β€” β€” Non-incubated control (in %), assay in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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