Cellobiohydrolase (gene MtCel6A) from Myceliophthora thermophila

Enzyme Description

Species
Myceliophthora thermophila β†—
Extremophile
Yes "thermophilic" organism
EC Number
3.2.1.91 β†—

Sequence

Length: 395 amino acids
MKFVQSATLAFAATALAAPSRTTPQKPRQASAGCASAVTLDASTNVFQQYTLHPNNFYRAEVEAAAEAISDSALAEKARKVADVGTFLWLDTIENIGRLEPALEDVPCENIVGLVIYDLPGRDCAAKASNGELKVGELDRYKTEYIDKIAEILKAHSNTAFALVIEPDSLPNLVTNSDLQTCQQSASGYREGVAYALKQLNLPNVVMYIDAGHGGWLGWDANLKPGAQELASVYKSAGSPSQVRGISTNVAGWNAWDQEPGEFSDASDAQYNKCQNEKIYINTFGAELKSAGMPNHAIIDTGRNGVTGLRDEWGDWCNVNGAGFGVRPTANTGDELADAFVWVKPGGESDGTSDSSAARYDSFCGKPDAFKPSPEAGTWNQAYFEMLLKNANPSF
Anica Dadwal et al. (2021) β€” Recombinant cellobiohydrolase of Myceliophthora thermophila: characterization and applicability in cellulose saccharification
AMB Express  Β· doi:10.1186/s13568-021-01311-8 β†—  Β· Activity - Classical
10 measurements ▼
Database ID
UniProt: G2QFW6 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (10 measurements)

10 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Acetone 10% 100 137 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent 1-Butanol 10% 100 100 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Chloroform 10% 100 94 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethanol 10% 100 103 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ethyl Acetate 10% 100 0 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent n-Hexane 10% 100 118 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isoamyl Alcohol 10% 100 133 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Isopropanol 10% 100 98 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Ammonia 10% 100 120 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number
Activity - Classical Activity measured by absorbance spectrophotometry (DNS assay, 540 nm) in the presence of organic solvent Methanol 10% 100 100 % 100 mM Tris-HCl buffer 10 60Β°C 0.5% Cellulose (Avicel PH-101) Reducing sugars β€” β€” Classical aqueous control (in %) | Deducted EC number

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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