Esterase B from Jatropha curcas

Enzyme Description

Species
Jatropha curcas β†—
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 204 amino acids
MVGVDFEIKNHAVKISQLEPGYRALIPDLYRGKVGLDVAEAQHLMEDLDWQSVVKDIRASVNWLKANGSKKVGVTGFCMGGALSIASSVLVPEVDAVVAFYGVPSSELADPAQAKAPIQAHFGELDNFVGFSDVTAAKALEEKLKASGIPYEVHIYPGSAHAFMNRSEEGIKRRKGMGMPDDNEAAAQLAWSRFSSWMGRYLSA
Marcos Gustavo Araujo Schwarz et al. (2021) β€” Revisiting Jatropha curcas Monomeric Esterase: A Dienelactone Hydrolase Compatible with the Electrostatic Catapult Model
Biomolecules  Β· doi:10.3390/biom11101486 β†—  Β· Activity - Classical Stability - Incubation
16 measurements ▼
Database ID
UniProt: A0A067JLI6 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Purified, eukaryote Jatropha curcas

Experimental Data (16 measurements)

16 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Isopropanol 10% 0.18 0.09 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Isopropanol 20% 0.18 0.08 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Isopropanol 40% 0.18 0.07 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Isopropanol 60% 0.18 0.03 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Acetonitrile 10% 0.18 0.07 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Acetonitrile 20% 0.18 0.05 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Acetonitrile 40% 0.18 0.02 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Acetonitrile 60% 0.18 0.0 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Ethanol 10% 0.18 0.12 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Ethanol 20% 0.18 0.03 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Ethanol 40% 0.18 0.01 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Activity - Classical Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in the presence of organic solvent Ethanol 60% 0.18 0.01 U/mL 100 mM Tris-HCl buffer 7.5 Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Classical aqueous control (in U/mL)
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in aqueous phase Isopropanol 10% 0.18 0.17 U/mL Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 7.5, Assay: 7.5 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in U/mL), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in aqueous phase Isopropanol 40% 0.18 0.18 U/mL Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 7.5, Assay: 7.5 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in U/mL), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in aqueous phase Acetonitrile 10% 0.18 0.18 U/mL Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 7.5, Assay: 7.5 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in U/mL), assay in aqueous phase | Clear about assay in aqueous phase
Stability - Incubation Activity after incubation (30 minutes at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 348 nm) in aqueous phase Acetonitrile 40% 0.18 0.17 U/mL Incubation: 100 mM Tris-HCl buffer, Assay: 100 mM Tris-HCl buffer Incubation: 7.5, Assay: 7.5 Incubation: Room temperature, Assay: Room temperature 1 mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” β€” Non-incubated control (in U/mL), assay in aqueous phase | Clear about assay in aqueous phase

Visualization : Activity β€” Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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