Esterase (gene est3S) from a cow rumen metagenomic library

Enzyme Description

Species
Na (cow rumen metagenomic library)
Extremophile
No
EC Number
3.1.1.1 β†—

Sequence

Length: 342 amino acids
MKEIIDYSKKENWYQIPEITKDVDTFYIYATEYILKSFDEGAPDFADLDNEEMLVGAAGEYLLHAATYADSTNVFMPYYRQVGLRYVGDVFKRDGIIDAAITGMPYDDILAAIDYYFEHYNNGRPFIIAGHSQGSVIIKYLLEKYFTKHPDYLQRMVAAYAIGYSFTKDYFDANPHLKFATGETDTGVIIAWNTEGPKNIEVNATNAVVLPGAISINPLNWKLDDTYAPASMNLGSIILNETTGELEIVDLGADAQVCPSRGVVVTHAKGEAMPEEAAKINEAFFGPEGRHGDDYSFFYNNIKVNVAKRVAAYMANAKTSADLVVYGRSSLPRTIRLSKPMR
Hee Yul Lee et al. (2021) β€” Mining of a novel esterase (est3S) gene from a cow rumen metagenomic library with organosphosphorus insecticides degrading capability: Catalytic insights by site directed mutations, docking, and molecular dynamic simulations
International Journal of Biological Macromolecules  Β· doi:10.1016/j.ijbiomac.2021.08.224 β†—  Β· Stability - Incubation
7 measurements ▼
Database ID
UniParc: UPI00279D66CF β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (7 measurements)

7 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetone 10% 100 39 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Diluted organic solvent in assay solution
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Acetonitrile 10% 100 29 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Diluted organic solvent in assay solution
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase 1-Butanol 10% 100 14 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Diluted organic solvent in assay solution
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Ethanol 10% 100 25 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Diluted organic solvent in assay solution
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase Methanol 10% 100 77 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Diluted organic solvent in assay solution
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase n-Hexane 10% 100 59 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Removal of organic solvent for assay solution
Stability - Incubation Activity measured after incubation (unclear duration, ? Β°C), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 420 nm) in aqueous phase 1-Propanol 10% 100 23 % Incubation: 50 mM sodium phosphate buffer, Assay: 50 mM sodium phosphate buffer Incubation: 7, Assay: 7 Incubation: ?, Assay: 40Β°C ? mM p-Nitrophenyl butyrate Butyric Acid , p-Nitrophenol β€” Incubation: 160 rpm Non-incubated control (in %), assay in aqueous phase | Diluted organic solvent in assay solution

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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