Catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3
Enzyme Description
Sequence
MSVKTMHTPEVKELLEKVAGLNQAGGNERVKTIVHRVMHDVFQIIEDLDITPEEFWSAVYYINELGKNGEAALLAPGLGMDKYLDIRMDAEDEQAGLAGGTPRTIEGPLYVAGAPESEGFARMDDGTQDGETMILTGVVTDQDGQPLPNTLVEVWHADLKGGYSYFDPSQSEYNLRRSIRTDANGRYTAQSIIPSGYGCPPEGATQALLNQLGRHGRRPAHIHYFVSQPGYKHLTTQINLAGDEYTYDDFAFATREELVCDAVRVDDAAAAEQYGLNQPFTKVEFNIQLVASDKPEHQQRHERKRALEDDIA
Jing Li et al. (2021)
β Expression and characterization of catechol 1,2-dioxygenase from Oceanimonas marisflavi 102-Na3
Protein Expression and Purification
Β· doi:10.1016/j.pep.2021.105964 β
Β· Stability - Incubation
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Database ID
UniParc: UPI000D30D9DF β
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)
Experimental Data (7 measurements)
7 measurements
| Property | Assay | Solvent | Solvent Volume | Aqueous Reference | Measured Value | Units | Solution | pH | Temperature | Substrate(s) | Product(s) | Cofactor(s) | Shaking | Comments |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | Methanol | 5% | 100.0 | 98.2 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | Ethanol | 5% | 100.0 | 102.4 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | n-Hexane | 5% | 100.0 | 87.5 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | n-Heptane | 5% | 100.0 | 82.1 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | Acetone | 5% | 100.0 | 80.3 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | Diethyl Ether | 5% | 100.0 | 24.6 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
| Stability - Incubation | Activity after incubation (1 hour at 30Β°C), measured by absorbance spectrophotometry (cis,cis-muconic acid absorbance measurement, 260 nm) in aqueous phase | Chloroform | 5% | 100.0 | 42.9 | % | Incubation: 50 mM Tris-HCl buffer, Assay: 50 mM Tris-HCl buffer | Incubation: 8, Assay: 8 | Incubation: 30Β°C, Assay: 30Β°C | ? mM Catechol | cis,cis-muconic acid | β | Incubation: 150 rpm | Non-incubated control (in %), assay in aqueous phase |
Visualization : Stability β Incubation
One bar per measurement. Colour = solvent, shade = solvent volume.