Serine protease SAPGB from Gracilibacillus boraciitolerans strain LO15

Enzyme Description

Species

Gracilibacillus boraciitolerans LO15 ↗

Extremophile

No but "moderately halotolerant" organism

EC Number

3.4.- ↗

Sequence

Length: 276 amino acids

EQTVPWRIDYIGKAAAHQKGWFGKGIRVGIIDSGIRPHKDLKVSGGINILDNSENYFDGYGHGTWYAGIIGALDNSYGLLGVASDADLYSIKVKKNDGKGLISDVVTGIEWAIENKMNIVNFSLQTNVETDILRKAIKKANENDILLVASSGNMGGTKKDDTVTYPAAYDEVISVAAINKNGERYVHSSTGKRIDISAPGELVYTTVQSGLYFTLNGTSMSAPHDSGVAALVLQNNPKLNIEEIRKILVKSTKPLGNPHLYGKGLIDVQKAIKFSN

Akli Ouelhadj et al. (2020) — Identification and homology modeling of a new biotechnologically compatible serine alkaline protease from moderately halotolerant Gracilibacillus boraciitolerans strain LO15

International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.07.266 ↗ · Activity - Classical

10 measurements ▼

Database ID

UniProt: A0A7L9CQD4 ↗

Sequence Annotation

Explicit - Provided (first 110 residues from UniProt sequence absent from the mature protein)

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3) pLys

Experimental Data (10 measurements)

10 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Decane	25%	100	79	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Octane	25%	100	101	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	25%	100	190	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	25%	100	70	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	25%	100	175	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	25%	100	88	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	25%	100	115	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetonitrile	25%	100	0	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	25%	100	103	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	129	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids , Peptides	—	—	Classical aqueous control (in %)

Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Decane	25%	100	79	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Octane	25%	100	101	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	n-Hexane	25%	100	190	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	25%	100	70	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Chloroform	25%	100	175	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	1-Butanol	25%	100	88	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Isopropanol	25%	100	115	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Acetonitrile	25%	100	0	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Methanol	25%	100	103	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)
Activity - Classical	Activity measured by absorbance spectrophotometry (colorimetric assay, Folin-Ciocalteu phenol reagant and free tyrosines reaction product absorbance measurement, 660 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	25%	100	129	%	83 mM glycine-NaOH buffer	10	60°C	0.8% Casein	free amino acids, Peptides	—	—	Classical aqueous control (in %)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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