Lipase 2 from Pseudomonas marinensis gcc21

Enzyme Description

Species
Pseudomonas marinensis gcc21 β†—
Extremophile
No but "cold-active" enzyme
EC Number
3.1.1.3 β†—

Sequence

Length: 293 amino acids
MNSPLLKLSTLTLGLVFAAGAYAFNPGNPGGPSNPGGDQPVESGFPSVGNFEASGSFATTNASEGPSCTIYRPRTLGENDLKHPIILWGNGTGTSPSGYSALLNHWASHGFVVAAANTTNAGSGAEMLACLDYLAEQNGRGSGTYAGKLNLDAVGSSGHSQGGGGSIMAGQDSRITATAPFQPYTLGLGHRRASQSNQNGPMFLMTGSADTLASSEANGRPVFNNANVPVFWGELQGASHFVPAFNGGGYRGPSTAWYRYHLMGDQNAESTFYGADCKLCTSSEWKVQKKGIQ
Chenchen Guo et al. (2021) β€” Characterization of Two Unique Cold-Active Lipases Derived from a Novel Deep-Sea Cold Seep Bacterium
Microorganisms  Β· doi:10.3390/microorganisms9040802 β†—  Β· Stability - Incubation
14 measurements ▼
Database ID
UniParc: UPI00145148D9 β†—
Sequence Annotation
Explicit - Provided
Protein Source
Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (14 measurements)

14 measurements
Property Assay Solvent Solvent Volume Aqueous Reference Measured Value Units Solution pH Temperature Substrate(s) Product(s) Cofactor(s) Shaking Comments
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Xylene 1% 4.6 0.26 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Xylene 10% 4.6 0.17 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 1% 4.6 2.33 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase 1-Butanol 10% 4.6 1.17 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 1% 4.6 1.05 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Dimethyl Sulfoxide (DMSO) 10% 4.6 1.0 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 1% 4.6 1.63 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Methanol 10% 4.6 1.5 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 1% 4.6 3.04 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Ethanol 10% 4.6 2.37 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 1% 4.6 3.61 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase Glycerol 10% 4.6 2.86 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase p-Xylene 1% 4.6 0.15 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase
Stability - Incubation Activity after incubation (1 hour at room temperature), measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 410 nm) in aqueous phase p-Xylene 10% 4.6 0.08 U/mg Incubation: ?, Assay: 40 mM Tris-HCl buffer, 10% isopropyl alcohol Incubation: ?, Assay: 8 Incubation: Room temperature, Assay: 4Β°C 1 mM p-Nitrophenyl palmitate p-Nitrophenol , Palmitic Acid β€” β€” Non-incubated control (in U/mg), assay in aqueous phase | Uncertainty about whether assay really was in aqueous phase

Visualization : Stability β€” Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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