Laccase-S from Trametes hirsuta

Enzyme Description

Species

Extremophile

EC Number

1.10.3.2 ↗

Sequence

Length: 238 amino acids

FQLNVIANMNNHTMLKQTSIHWHCHFQKGTNWADGHAFVNACPIASGHSFLYDFTAPDQHGTFWYHSHLSTQYCDGLRGHFVVYDPADPHHDLYDVDDEHTIITLADWYHVAAKLGHHFQLGADSTLINGSGRFAGDPTAHLTVIYVTQGKRYRFHLVSLSCDPNHVFSIDSNHMTVIEADAVSHEHCTVDSIQIYAGQRYSFHLTVDQDVDNYWIRAHPSFGTYSFHDGINSAIARY

Jing Si et al. (2021) — Introducing a Thermo-Alkali-Stable, Metallic Ion-Tolerant Laccase Purified From White Rot Fungus Trametes hirsuta

Frontiers in Microbiology · doi:10.3389/fmicb.2021.670163 ↗ · Stability - Incubation

14 measurements ▼

Database ID

UniProt: C0HLV6 ↗

Sequence Annotation

Explicit - Provided GenBank Accession Number

Protein Source

Purified, fungus Trametes hirsuta

Experimental Data (14 measurements)

14 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	95.32	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	30%	100.0	81.43	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	90.54	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	30%	100.0	84.32	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	87.47	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	30%	100.0	79.56	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	10%	100.0	92.78	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	30%	100.0	76.26	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	62.18	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	30%	100.0	50.06	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Toluene	10%	100.0	135.05	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Toluene	30%	100.0	167.98	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Chloroform	10%	100.0	85.37	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Chloroform	30%	100.0	70.51	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase

Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	10%	100.0	95.32	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Methanol	30%	100.0	81.43	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	10%	100.0	90.54	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Ethanol	30%	100.0	84.32	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	10%	100.0	87.47	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	1-Propanol	30%	100.0	79.56	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	10%	100.0	92.78	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	n-Hexane	30%	100.0	76.26	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	10%	100.0	62.18	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Acetone	30%	100.0	50.06	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Toluene	10%	100.0	135.05	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Toluene	30%	100.0	167.98	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Chloroform	10%	100.0	85.37	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase
Stability - Incubation	Activity after incubation (20 minutes at 50°C), measured by absorbance spectrophotometry (ABTS radical cation reaction products absorbance measurement, 420 nm) in aqueous phase	Chloroform	30%	100.0	70.51	%	Incubation: ?, Assay: 50 mM sodium tartrate buffer	Incubation: ?, Assay: 4	Incubation: 50°C, Assay: Room temperature	0.5 mM ABTS	ABTS radical cation	—	—	Water-incubated control (in %), assay in aqueous phase \| Uncertainty about whether assay really was in aqueous phase

Visualization : Stability — Incubation

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

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