Protease (gene Apr‐BO1) from Bacillus pumilus BO1

Enzyme Description

Species

Bacillus pumilus BO1 ↗

Extremophile

No but "psychrotrophic" organism and "cold-adapted" lipase

EC Number

3.4.- ↗

Sequence

Length: 346 amino acids

GFGGSMANAETVSKTDSEKSYIVGFKASATTNSSKKQAVIQNGGKLEKQYRLINAAQVKMSEQAAKKLEHDPSIAYVEEDHKAEAYAQTVPYGIPQIKAPAVHAQGYKGANVKVAVLDTGIHAAHPDLNVAGGASFVPSEPNATQDFQSHGTHVAGTIAALDNTIGVLGVAPSASLYAVKVLDRYGDGQYSWIISGIEWAVANNMDVINMSLGGPSGSTALKNAVDTANNRGVVVVAAAGNSGSSGSSSTVGYPAKYDSTIAVANVNSNNVRNSSSSAGPELDVSAPGTSILSTVPSSGYTSYTVTSMASPHVAGAAALILSKNPNLTNSQVRQRLENTATPLGDS

Saleem Farooq et al. (2021) — Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

Scientific Reports · doi:10.1038/s41598-021-92197-w ↗ · Activity - Classical Stability - Incubation

6 measurements ▼

Database ID

UniProt: A0A7S9C359 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Purified, bacterium Bacillus pumilus BO1

Experimental Data (6 measurements)

6 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in the presence of organic solvent	Benzene	0.5%	100.0	86.97	%	100 mM Tris-Hcl buffer	9	20°C	0.6% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about pH and solvent volume
Activity - Classical	Activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	0.5%	100.0	95.1	%	100 mM Tris-Hcl buffer	9	20°C	0.6% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about pH and solvent volume
Activity - Classical	Activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in the presence of organic solvent	Xylene	0.5%	100.0	100.73	%	100 mM Tris-Hcl buffer	9	20°C	0.6% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about pH and solvent volume
Stability - Incubation	Activity after incubation (30 minutes at 20°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in aqueous phase	Benzene	0.5%	100.0	79.53	%	Incubation: 100 mM Tris-Hcl buffer, Assay: 100 mM Tris-Hcl buffer	Incubation: 9, Assay: 9	Incubation: 20°C, Assay: 20°C	0.6% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really is in aqueous phase, uncertainty about control, uncertainty about pH and solvent volume
Stability - Incubation	Activity after incubation (30 minutes at 20°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in aqueous phase	Toluene	0.5%	100.0	96.47	%	Incubation: 100 mM Tris-Hcl buffer, Assay: 100 mM Tris-Hcl buffer	Incubation: 9, Assay: 9	Incubation: 20°C, Assay: 20°C	0.6% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really is in aqueous phase, uncertainty about control, uncertainty about pH and solvent volume
Stability - Incubation	Activity after incubation (30 minutes at 20°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in aqueous phase	Xylene	0.5%	100.0	92.97	%	Incubation: 100 mM Tris-Hcl buffer, Assay: 100 mM Tris-Hcl buffer	Incubation: 9, Assay: 9	Incubation: 20°C, Assay: 20°C	0.6% Casein	free amino acids , Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really is in aqueous phase, uncertainty about control, uncertainty about pH and solvent volume

Activity - Classical	Activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in the presence of organic solvent	Benzene	0.5%	100.0	86.97	%	100 mM Tris-Hcl buffer	9	20°C	0.6% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about pH and solvent volume
Activity - Classical	Activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in the presence of organic solvent	Toluene	0.5%	100.0	95.1	%	100 mM Tris-Hcl buffer	9	20°C	0.6% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about pH and solvent volume
Activity - Classical	Activity measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in the presence of organic solvent	Xylene	0.5%	100.0	100.73	%	100 mM Tris-Hcl buffer	9	20°C	0.6% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about pH and solvent volume
Stability - Incubation	Activity after incubation (30 minutes at 20°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in aqueous phase	Benzene	0.5%	100.0	79.53	%	Incubation: 100 mM Tris-Hcl buffer, Assay: 100 mM Tris-Hcl buffer	Incubation: 9, Assay: 9	Incubation: 20°C, Assay: 20°C	0.6% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really is in aqueous phase, uncertainty about control, uncertainty about pH and solvent volume
Stability - Incubation	Activity after incubation (30 minutes at 20°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in aqueous phase	Toluene	0.5%	100.0	96.47	%	Incubation: 100 mM Tris-Hcl buffer, Assay: 100 mM Tris-Hcl buffer	Incubation: 9, Assay: 9	Incubation: 20°C, Assay: 20°C	0.6% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really is in aqueous phase, uncertainty about control, uncertainty about pH and solvent volume
Stability - Incubation	Activity after incubation (30 minutes at 20°C), measured by absorbance spectrophotometry (free tyrosines absorbance measurement, 660 nm) in aqueous phase	Xylene	0.5%	100.0	92.97	%	Incubation: 100 mM Tris-Hcl buffer, Assay: 100 mM Tris-Hcl buffer	Incubation: 9, Assay: 9	Incubation: 20°C, Assay: 20°C	0.6% Casein	free amino acids, Peptides	—	—	Non-incubated control (in %), assay in aqueous phase \| Uncertainty about whether the assay really is in aqueous phase, uncertainty about control, uncertainty about pH and solvent volume

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Visualization : Stability — Incubation