Esterase DS-007 from a hot spring metaproteomic library

Enzyme Description

Species

Na (hot spring metaproteomic library)

Extremophile

Yes organism from hot spring, thermophilic enzyme

EC Number

3.1.1.1 ↗

Sequence

Length: 215 amino acids

MNVHTEERLIPGPVGQIDLSIDQPDTALRGLALIGHPHPLFGGTKDNKVAQTLARTFVGLGYATVRLNFRGVGKTEGTHDNGIGEQDDMLAVLDWMRTQTEWSADVATLPLALGGFSFGSFVVSQVARRLAEAGTPAERLALVGTATSRWDVAPVPADTIIIHGEQDDTVPLIDVLNWARPQELPVIVIPGADHFFHRKLHLIRQLITGAWAPKP

Dennis Sander et al. (2021) — Metaproteomic Discovery and Characterization of a Novel Lipolytic Enzyme From an Indian Hot Spring

Frontiers in Microbiology · doi:10.3389/fmicb.2021.672727 ↗ · Activity - Classical

15 measurements ▼

Database ID

UniProt: A0ABM9IQU0 ↗

Sequence Annotation

Explicit - Provided

Protein Source

Recombinant, host bacterium Escherichia coli BL21 (DE3)

Experimental Data (15 measurements)

15 measurements

Property	Assay	Solvent	Solvent Volume	Aqueous Reference	Measured Value	Units	Solution	pH	Temperature	Substrate(s)	Product(s)	Cofactor(s)	Shaking	Comments
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	1%	16.7	24.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	16.7	8.4	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	16.7	0.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	16.7	13.4	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	16.7	8.2	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	16.7	3.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	16.7	9.3	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	16.7	0.2	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	16.7	0.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	1%	16.7	11.9	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	16.7	8.7	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	16.7	0.1	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	1%	16.7	11.2	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	16.7	0.6	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	16.7	0.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid , p-Nitrophenol	—	—	Classical aqueous control (in micromol * microgram^-1 * min^-1)

Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	1%	16.7	24.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	10%	16.7	8.4	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Methanol	20%	16.7	0.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	1%	16.7	13.4	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	10%	16.7	8.2	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethyl Sulfoxide (DMSO)	20%	16.7	3.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	1%	16.7	9.3	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	10%	16.7	0.2	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Dimethylformamide (DMF)	20%	16.7	0.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	1%	16.7	11.9	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	10%	16.7	8.7	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Isopropanol	20%	16.7	0.1	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	1%	16.7	11.2	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	10%	16.7	0.6	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)
Activity - Classical	Activity measured by absorbance spectrophotometry (p-nitrophenol absorbance measurement, 405 nm) in the presence of organic solvent	Acetone	20%	16.7	0.0	µmol * µgram^-1 * min^-1	50 mM sodium phosphate buffer assay	9.5	55°C	? mM p-Nitrophenyl butyrate	Butyric Acid, p-Nitrophenol	—	—	Classical aqueous control (in µmol * µgram^-1 * min^-1)

Visualization : Activity — Classical

One bar per measurement. Colour = solvent, shade = solvent volume.

Structure

Drag to rotate · Scroll to zoom · Right-click drag to pan · Powered by Mol*